New insights into the influence of myo-inositol on carbohydrate metabolism during osmoregulation in Nile tilapia (Oreochromis niloticus)  

作　　者：Jiahua Zhu Liqiao Chen Yuxing Huang Fan Zhang Jingyu Pan Erchao Li Jianguang Qin Chuanjie Qin Xiaodan Wang 

机构地区：[1]Laboratory of Aquaculture Nutrition and Environmental Health,School of Life Sciences,East China Normal University,Shanghai 200241,China [2]College of Science and Engineering,Flinders University,Adelaide,SA 5001,Australia [3]Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province,Hainan Aquaculture Breeding Engineering Research Center,College of Marine Sciences,Hainan University,Haikou 570228,China [4]Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River,Neijiang Normal University,Neijiang 641100,China

出　　处：《Animal Nutrition》2022年第3期86-98,共13页动物营养（英文版）

基　　金：grants from the National Natural Science Foundation of China(No.32172946);China Postdoctoral Science Foundation(2018 M630418);the Fundamental Research Funds for the Central Universities,ECNU and China Agriculture Research System-46(CARS-46).

摘　　要：A two-factor(23)orthogonal testwas conducted to investigate the effects of dietary myo-inositol(MI)on the osmoregulation and carbohydrate metabolism of euryhaline fish tilapia(Oreochromis niloticus)under sustained hypertonic stress(20 practical salinity units[psu]).6 diets containing either normal carbohydrate(NC,30%)or high carbohydrate(HC,45%)levels,with 3 levels(0,400 and 1,200 mg/kg diet)of MI,respectively,were fed to 540 fish under 20 psu for 8 weeks.Dietary MI supplementation significantly improved growth performance and crude protein content of whole fish,and decreased the content of crude lipid of whole fish(P<0.05).Curled,disordered gill lamella and cracked gill filament cartilage were observed in the gill of fish fed diets without MI supplementation.The ion transport capacity in gill was significantly improved in the 1,200 mg/kg MI supplementation groups compared with the 0 mg/kg MI groups(P<0.05).Moreover,the contents of Na^(+),K^(+),Cl^(-)in serum weremarkedly reduced with the dietary MI supplementation(P<0.05).The fish fed 1,200 mg/kg MI supplementation had the highest MI content in the gills and the lowest MI content in the serum(P<0.05).Additionally,the fish fed with 1,200 mg/kg MI supplementation had the highest MI synthesis capacity in gills and brain(P<0.05).Dietary MI markedly promoted the ability of carbohydrate metabolism in liver(P<0.05).Moreover,fish in the 1,200 mg/kg MI groups had the highest antioxidant capacity(P<0.05).This study indicated that high dietary carbohydrate would intensify stress,and impair the ability of osmoregulation in tilapia under a long-term hypersaline exposure.The supplementation of MI at 1,200 mg/kg in the high carbohydrate diet could promote carbohydrate utilization and improve the osmoregulation capacity of tilapia under long-term hypertonic stress.

关 键 词：MYO-INOSITOL Carbohydrate metabolism OSMOREGULATION Oreochromis niloticus 

分 类 号：S965.125[农业科学—水产养殖]