响应面法优化九头狮子草中绿原酸提取工艺  

Optimization of extraction technology of chlorogenic acid from Peristrophe japonica by response surface methodology

作　　者：高林晓 邓晓霞 郭蒙 彭莘媚 GAO Lin-xiao;DENG Xiao-xia;GUO Meng;PENG Xin-mei

机构地区：[1]黔南民族师范学院化学化工学院,贵州黔南558000 [2]贵州省普通高校民族药用植物资源开发工程研究中心,贵州黔南558000

出　　处：《饲料研究》2022年第17期75-79,共5页Feed Research

基　　金：贵州省教育厅青年科技人才成长项目(项目编号:黔教合KY字[2018]440、[2015]389);黔南民族师范学院项目(项目编号:2020qnsy14、qnsycxyjd2018005、qnsyk201603);贵州省普通高校民族药用植物资源开发工程研究中心开放基金(项目编号:黔教合KY字[2014]227)。

摘　　要：试验旨在使用响应面法优化九头狮子草中绿原酸提取工艺。试验以九头狮子草为原料,乙醇为溶剂,以九头狮子草中绿原酸的提取率为评价指标,单因素试验为基础,采用响应面法优选出九头狮子草中绿原酸提取率的最佳工艺。结果显示:最优工艺条件为乙醇体积分数40%、液料比25 mL/g、超声时间80 min、超声温度50℃,此工艺条件下绿原酸提取率为1.82%。研究表明,此提取工艺操作简单、效果良好,可为九头狮子草中绿原酸进一步纯化提取提供参考。The study was to optimize the extraction process of chlorogenic acid from Peristrophe japonica by response surface methodology.The experiment used Peristrophe japonica as raw material,ethanol as solvent,and extraction rate of chlorogenic acid in Peristrophe japonica as an evaluation index,based on single factor test,using response surface methodology to optimize optimal process for extraction rate of chlorogenic acid in Peristrophe japonica.The results showed that the optimal process conditions were determined as ethanol volume fraction 40%,liquid tosolid ratio 25 mL/g,ultrasonic time 80 min,ultrasonic temperature 50℃,and the extraction rate of chlorogenic acid was 1.82%.The study indicates that the extraction process is simple and effective,which can provide a reference for the further purification and extraction of chlorogenic acid in Peristrophe japonica.

关 键 词：九头狮子草 绿原酸 超声提取 响应面试验 

分 类 号：S816.7[农业科学—饲料科学]