刺五加茎皮总黄酮的抗氧化活性研究  

Study on antioxidant activity of total flavonoids from Acanthopanax senticosus stem bark

作　　者：苏建青[1] 王晓娅 张馨予 高腾 付祥 李晓丽 薛娇娇 褚秀玲[1] SU Jian-qing;WANG Xiao-ya;ZHANG Xin-yu;GAO Teng;FU Xiang;LI Xiao-li;XUE Jiao-jiao;CHU Xiu-ling

机构地区：[1]聊城大学农学与农业工程学院,山东聊城252000

出　　处：《饲料研究》2022年第17期80-84,共5页Feed Research

基　　金：国家自然科学基金(项目编号:31872515、32172901)。

摘　　要：试验旨在评估刺五加茎皮总黄酮的抗氧化能力。通过体外抗氧化试验和建立鸡胚成纤维细胞(CEF)氧化损伤模型,评价刺五加茎皮总黄酮的抗氧化能力。结果显示:刺五加总黄酮具有较强的清除体外氧化自由基的能力,对氧化损伤的CEF细胞具有保护作用,调节氧化损伤引起的总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的改变。研究表明,刺五加茎皮总黄酮具有较强的抗氧化活性。The experiment was to evaluate the antioxidant capacity of total flavonoids of stem bark of Acanthopanax senticosus.The in vitro antioxidant assay and the establishment of a chicken embryonic fibroblast(CEF)oxidative damage model were used to evaluate the antioxidant capacity of total flavonoids from stem bark of Acanthopanax senticosus.The results showed that total flavonoids of Acanthopanax stoloniferum had strong ability to scavenge oxidative free radicals in vitro,and had protective effect on oxidatively damaged CEF cells,regulating the changes of T-AOC,SOD,GSH-Px activity and MDA content caused by oxidative damage.The study indicates that the total flavonoids of stem bark of Acanthopanax senticosus has strong antioxidant activity.

关 键 词：刺五加 总黄酮 鸡胚成纤维细胞 氧化损伤 

分 类 号：S816.7[农业科学—饲料科学]