隔药饼灸对高脂血症兔IGF-1/Sp1蛋白及基因表达的影响  

Effects of herbal cake-separated moxibustion on IGF-1/Sp1 protein and gene expression in hyperlipidemia rabbits

作　　者：李芊 欧阳里知 刘惠娟 彭涵 刘红华[2] 葛君芸[1] 常小荣[1] 刘迈兰[1] LI Qian;OUYANG Lizhi;LIU Huijuan;PENG Han;LIU Honghua;GE Junyun;CHANG Xiaorong;LIU Mailan(College of Acu-moxibustion and Massage,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China;Nursing College,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China)

机构地区：[1]湖南中医药大学针灸推拿学院,湖南长沙410208 [2]湖南中医药大学护理学院,湖南长沙410208

出　　处：《湖南中医药大学学报》2022年第10期1688-1694,共7页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金项目(82074559);湖南省自然科学基金项目(2018JJ3097,2020JJ5414,2021JJ40401);湖南省院士专家工作站(石学敏)开放基金项目(2019YSZJJ10);湖南省教育厅优秀青年基金项目(19B435,19B428);长沙市杰出创新青年培养计划项目(kq1905036);湖南省大学生研究性学习和创新性实验计划(2018-15)。

摘　　要：目的探究隔药饼灸对高脂血症兔胰岛素样生长因子1(insulin-like growth factor 1,IGF-1)/特异性蛋白1(specificity protein 1,Sp1)蛋白及基因表达的影响。方法将36只雄性新西兰兔随机分为正常组、模型组和隔药饼灸组,每组12只。造模各组予高胆固醇配方饲料喂养8周制备高脂血症模型,正常组普食饲养。模型组捆绑操作,隔药饼灸组隔药饼灸干预,施灸穴位分为腹背两组,腹组为“巨阙”与双侧“天枢”“丰隆”,背组为双侧“心俞”“肝俞”“脾俞”,每天只施灸一组穴位,两组穴位交替施灸,持续8周。干预8周后,取各组兔血清、主动脉组织及肝组织样本,采用ELISA、RT-PCR技术检测IGF-1、Sp1指标。结果(1)干预8周后,与正常组比较,模型组血清IGF-1、Sp1含量上升(P<0.01);与模型组比较,隔药饼灸组血清IGF-1、Sp1含量下降(P<0.01)。(2)与正常组比较,模型组主动脉IGF-1的mRNA表达量显著上升(P<0.001),与模型组比较,隔药饼灸组主动脉IGF-1的mRNA表达量下降(P<0.01)。(3)与正常组比较,模型组肝组织IGF-1和Sp1的mRNA表达量显著上升(P<0.001,P<0.01);与模型组比较,隔药饼灸组肝组织IGF-1和Sp1的mRNA表达量下降(P<0.01)。结论隔药饼灸可能影响IGF-1/Sp1信号通路,通过胆固醇逆转运环节,调节LDL-C的摄取和转运而缓解高脂血症。Objective To investigate the effects of herbal cake-separated moxibustion on the expressions of insulin-like growth factor 1(IGF)/specificity protein 1(Sp1)protein and gene expression in rabbits with hyperlipidemia.Methods A total of 36 male New Zealand rabbits were randomly divided into normal group,model group and herbal cake-separated moxibustion group,with 12 rabbits in each.The hyperlipidemia models were prepared in each modeling group by feeding with high cholesterol formula for 8 weeks.The normal group was fed with common food;the model group was bundled;and the herbal cake-separated moxibustion group was treated with herbal cake-separated moxibustion with the applied acupoints divided into the abdominal and dorsal groups."Juque"(CV14),bilateral"Tianshu"(S25)and“Fenglong”(S40)were chosen for the abdominal groups,and bilateral"Xinshu"(BL15),“Ganshu”(BL18)and“Pishu”(BL20)for the back group.Moxibustions were applied only to one group of acupoints per day,and the two groups of acupoints were alternately applied for 8 weeks.After 8 weeks of intervention,serum,aortic tissue and liver tissue samples were taken from each group,and the IGF-1/Sp1 indicators were detected by ELISA,RT-PCR techniques.Results(1)After 8 weeks of intervention,compared with the normal group,the levels of serum IGF-1/Sp1 increased in the model group(P<0.01);compared with the model group,the serum IGF-1/Sp1 content in the herbal cake-separated moxibustion group decreased(P<0.01).(2)Compared with the normal group,the mRNA expression of IGF-1 in the aorta of the model group was significantly reduced(P<0.001).Compared with the model group,the mRNA expression of IGF-1 in the aorta of the herbal cake-separated moxibustion group decreased(P<0.01).(3)Compared with the normal group,the mRNA expression of IGF-1/Sp1 in liver tissues of the model group increased significantly(P<0.001,P<0.01);compared with the model group,the mRNA expression levels of IGF-1/Sp1 in liver tissues of herbal cake-separated moxibustion group decreased(P<0.01).Con

关 键 词：高脂血症 隔药饼灸 胰岛素样生长因子1 特异性蛋白1 胆固醇逆转运 低密度脂蛋白胆固醇 IGF-1/Sp1信号通路 血脂 

分 类 号：R245.8[医药卫生—针灸推拿学]