不同浓度次氯酸钠处理芹菜种子对其萌发和幼苗质量的影响  

Effects of Different Concentrations of Sodium Hypochlorite on Seeds Germination and Seedlings Quality of Celery

作　　者：谭杉杉 仇亮 段奥其 束胜 曾晓萍 朱为民[5] 贾敏 阎君[5] 刘燕花 刘慧 熊爱生[1] TAN Shanshan;QIU Liang;DUAN Aoqi;SHU Sheng;ZENG Xiaoping;ZHU Weimin;JIA Min;YAN Jun;LIU Yanhua;LIU Hui;XIONG Aisheng(State Key Laboratory of Crop Genetics and Germplasm Enhancement,College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;Suqian Academy of Protected Horticultures,Suqian,Jiangsu 223800,China;Institute of Agricultural Sciences in Riverside Region of Jiangsu,Nantong,Jiangsu 226001,China;Jiangsu Agricultural Technical Extension Station,Nanjing 210036,China;Horticulture Research Institute,Shanghai Academy Agricultural Sciences,Shanghai 201403,China)

机构地区：[1]南京农业大学园艺学院,作物遗传与种质创新国家重点实验室,南京210095 [2]宿迁市设施园艺研究院,江苏宿迁223800 [3]江苏沿江地区农业科学研究所,江苏南通226001 [4]江苏省农业技术推广总站,南京210036 [5]上海市农业科学院设施园艺研究所,上海201403

出　　处：《园艺学报》2022年第9期1907-1921,共15页Acta Horticulturae Sinica

基　　金：江苏省种业振兴揭榜挂帅项目(JBGS2021-068);江苏省重点研发计划现代农业项目(BE2022386);宿迁市重点研发计划项目(L202108);国家自然科学基金项目(31272175);江苏高校优势学科建设项目(PAPD)。

摘　　要：芹菜种子小,且含有大量的萌发抑制物质,给其种苗繁育带来困难。采用培养皿培养和穴盘培养两种方式,用不同浓度(0、5%、10%、20%、40%)NaClO处理‘六合黄心芹’和‘赛雪’芹菜种子,对种子萌发指标、幼苗生长指标及光合参数进行测定,并分析幼苗中叶绿素含量变化以及相关基因表达。结果表明:5%次氯酸钠溶液处理芹菜种子,其发芽率、发芽势、发芽指数和活力指数最高,幼苗生长势最强,幼苗的壮苗指标、叶绿素含量以及净光合速率也有一定程度提高,相关基因的相对表达量也较高。这些结果说明次氯酸钠处理促进芹菜种子的适宜浓度为5%。Celery seeds are small and contain a large amount of germination-inhibiting substances,which brings difficulties to celery seeding propagation. The seeds of‘Liuhe Huangxinqin’and‘Saixue’were treated with different concentrations(0,5%,10%,20%,40%)of NaClO cultured in petri dish and acupoint dish. The seed germination index,seedling growth index and photosynthetic parameters were measured. In addition,the changes of chlorophyll content and related gene expression in seedlings were analyzed. The results showed that celery seeds treated with 5% NaClO had the highest germination rate,germination potential,germination index and vigor index,and the strongest seedling growth potential. And the seedling vigor index,chlorophyll content and net photosynthetic rate were also increased to some extent. Also,the relative expression levels of related genes were also higher. These results suggested that the appropriate concentration for NaClO to promote celery seeds is 5%.

关 键 词：芹菜 种子 次氯酸钠 萌发 幼苗 叶绿素 

分 类 号：S636.3[农业科学—蔬菜学]