宽带微量太赫兹辐射促进神经元生长发育  

Neuronal growth and development promoted by low-intensity roadband terahertz radiation

作　　者：马少卿 龚士香 张微 路承彪[3] 李小俚[4] 李英伟[1,2] Ma Shao-Qing;Gong Shi-Xiang;Zhang Wei;Lu Cheng-Biao;Li Xiao-Li;Li Ying-Wei(School of Information Science and Engineering,Yanshan University,Qinhuangdao 066004,China;Hebei Key Laboratory of Information Transmission and Signal Processing,Qinhuangdao 066004,China;Henan International Key Laboratory for Noninvasive Neuromodulation,Xinxiang Medical University,Xinxiang 453000,China;State Key Laboratory of Cognitive Neuroscience and Learning,Beijing Normal University,Beijing 100875,China)

机构地区：[1]燕山大学信息科学与工程学院,秦皇岛066004 [2]河北省信息传输与信号处理重点实验室,秦皇岛066004 [3]新乡医学院河南省无创神经调控国际重点实验室,新乡453000 [4]北京师范大学认知神经科学与学习国家重点实验室,北京100875

出　　处：《物理学报》2022年第20期341-351,共11页Acta Physica Sinica

基　　金：国家自然科学基金(批准号:61827811);河北省自然科学基金(批准号:F2020203099);河北省重点实验室项目(批准号:202250701010046);河北省引进留学人员项目(批准号:C20200364)资助的课题。

摘　　要：太赫兹波位于氢键和范德瓦耳斯力作用能级范围内,可以直接与蛋白质耦合激发蛋白质的非线性共振效应,从而影响蛋白质的构象、神经元的结构和功能.基于此机制,体外培养SD大鼠原代皮层神经元,利用宽带微量太赫兹(0.3—3.0 THz,最大辐射功率100μW)短时间累计辐射(3 min/d,共3 d)皮层神经元;记录皮层神经元的动态发育参数(胞体面积和突起总长度);并分析辐射结束后神经元受体相关蛋白(GluA1和GluN1)、突触素(SY-38)和突触后致密蛋白-95(PSD-95)的表达变化.太赫兹辐射1 d后,神经元胞体面积增长值提高了144.9%(P<0.05);太赫兹辐射的2 d和3 d后,神经元突起总长度增长值分别提高了65.1%(P<0.05)和109.4%(P<0.05);太赫兹辐射3 d后,GluA1和SY-38蛋白表达分别提高了38.1%(P<0.05)和19.2%(P<0.05).结果表明,宽带微量太赫兹短时累计辐射可以促进皮层神经元胞体和突起的生长,并且对神经元突起的促进作用存在累计效应;太赫兹辐射对神经元生长发育的促进作用可能与GluA1和SY-38蛋白表达相关.这些结果预示着特定频率和能量的太赫兹波可以发展为一种治疗或干预神经发育障碍等疾病的新型神经调控技术.Terahertz waves are located in the energy level range of hydrogen bonding and van der Waals forces,and can directly couple with proteins to excite the nonlinear resonance effect of proteins.Therefore,terahertz wave can affect the conformation of proteins,the structure and function of neurons.Primary cerebral cortex neurons of SD rats are cultured in vitro.Neurons are radiated 3 days by THz wave with 0.3-3.0 THz in frequency and 100μW in power;the growth and development indicators of neurons(Cell body area,total length of process)are recorded.At the end of a radiation programme,Western blotting is used to detect the protein expressions of GluA1,GluN1,postsynaptic density protein-95(PSD-95)and synaptophysin 38(SYP-38).After the first day of terahertz wave radiation,the cell area is increased by 144.9%(P<0.05);on the second day and third day of terahertz wave radiation,the growth value of the total length of neuronal neurites are increased by 65.1%(P<0.05)and 109.4%(P<0.05),respectively.After the three-day terahertz wave irradiation,the protein expressions of GluA1 and SY-38 are increased by 38.1%(P<0.05)and 19.2%(P<0.05),respectively.In addition,some results show below.1)The use of low-intensity broadband terahertz wave in this study will not cause the cortical neurons to die,and will not affect their regular growth.2)Low-intensity broadband terahertz radiation can promote the growth of cortical neuron cell bodies and processes,but the effects on cortical neuron cell bodies and processes are different.This may be related to the developmental cycle of cultured cortical neurons in vitro,and there is a cumulative effect on the promotion of neuronal processes by low-intensity broadband terahertz.3)The promotion of neuronal growth and development by low-intensity broadband terahertz wave radiation may be related to the proportion of AMPA receptor subtypes and the expression of presynaptic specific protein SY-38.These results herald a specific-frequency and specific-energy terahertz radiation can be developed into a nov

关 键 词：太赫兹 神经元 生长发育 累计效应 

分 类 号：O441.4[理学—电磁学] Q42[理学—物理]