雌二醇联合白藜芦醇对去卵巢骨质疏松大鼠骨密度和生物力学的影响  

Effects of estradiol combined with resveratrol on bone mineral density and biomechanics in ovariectomized osteoporosis rats

作　　者：张海涛 黄一拯 李超 黄勇 ZHANG Hai-tao;HUANG Yi-zheng;LI Chao(Department of Spine Surgery,Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan Hubei 430000,China)

机构地区：[1]湖北省中医院脊柱外科,湖北武汉430000

出　　处：《临床和实验医学杂志》2022年第18期1909-1912,共4页Journal of Clinical and Experimental Medicine

基　　金：湖北省卫生健康委员会项目(编号:WJ2021M182)。

摘　　要：目的探究雌二醇联合白藜芦醇对去卵巢骨质疏松大鼠骨密度和生物力学的影响。方法将50只6周龄SPF级雌性SD大鼠按随机数字表法分为假手术组、模型组、雌二醇组、白藜芦醇组、联合组,每组各10只。检测各组大鼠处理后骨矿含量和骨密度。收集大鼠血清,检测大鼠血清中代谢指标骨钙素、骨桥蛋白和抗酒石酸酸性磷酸酶(TRAP)含量。对比各组大鼠弹性模量和断裂载荷等生物力学相关指标。结果与假手术组大鼠相比,其余各组大鼠骨矿含量、骨密度、血清中骨钙素和骨桥蛋白含量、弹性模量以及断裂载荷均显著降低,血清中TRAP含量显著升高,差异均有统计学意义(P<0.05);与模型组大鼠相比,雌二醇组、白藜芦醇组和联合组大鼠骨矿含量、骨密度、血清中骨钙素和骨桥蛋白含量、弹性模量以及断裂载荷均显著升高,血清中TRAP含量显著降低,差异均有统计学意义(P<0.05);与雌二醇组和白藜芦醇组大鼠相比,联合组大鼠各指标均进一步改善,差异均有统计学意义(P<0.05)。结论雌二醇联合白藜芦醇对去卵巢骨质疏松大鼠的骨密度和生物力学改善作用明显。Objective To investigate the biomechanical effects of resveratrol and resveratrol on osteoporosis in rats.Methods Fifty 6-week-old SPF female SD rats were divided into five groups with ten rats in each group according to the random number table method,sham operation group,model group,estradiol group,resveratrol group and combined group.Bone mineral content(BMC)and bone mineral density(BMD)were measured after treatment.The contents of osteocalcin(OCN),osteopontin(OPN)and tartrate resistant acid phosphatase(TRAP)were measured.The biomechanical indexes such as elastic modulus and breaking load of rats in each group were compared.Results Compared with the sham operation group,the BMC,BMD,the contents of OCN and OPN in serum,elastic modulus and breaking load in other groups were significantly reduced,and the content of trap in serum was significantly increased,the differences were statistically significant(P<0.05).Compared with the model group,BMC,BMD,serum OCN and OPN contents,elastic modulus and breaking load in estradiol group,resveratrol group and combined group were significantly increased,and the content of trap in serum was significantly decreased,the differences were statistically significant(P<0.05).Compared with estradiol group and resveratrol group,all indexes in the combined group were further improved,the differences were statistically significant(P<0.05).Conclusion Estradiol combined with resveratrol can significantly improve bone mineral density and biomechanics in ovariectomized osteoporosis rats.

关 键 词：大鼠 去卵巢骨质疏松 雌二醇 白藜芦醇 骨密度 生物力学 

分 类 号：R580[医药卫生—内分泌]