Evaluation of thermostable endoglucanase in Paenibacillus lautus strain BHU3 for yield enhancement  

作　　者：Suman Yadav Anand Kumar Pandey Suresh Kumar Dubey 

机构地区：[1]Molecular Ecology Laboratory,Centre of Advanced Study in Botany,Institute of Science,Banaras Hindu University,Varanasi 221005,India [2]Department of Biotechnology Engineering,Institute of Engineering and Technology,Bundelkhand University,Jhansi 284128,India

出　　处：《Systems Microbiology and Biomanufacturing》2022年第4期607-622,共16页系统微生物学与生物制造（英文）

基　　金：This research work was financially assisted by University Grants Commission,Government of India,New Delhi,in the form of Junior and Senior Research Fellowship to Suman(Grant No.09/013(0617)/2016-EMR-I).

摘　　要：In the present study,Paenibacillus lautus strain BHU3 isolated from landfill soil was evaluated for the presence of potential endoglucanases which are the first candidate of cellulase enzyme system to act on cellulose.In-silico analysis revealed high potential thermostable endoglucanases which can efficiently interact with cellulose.The most potent and thermostable endoglucanase(locus tag id.CPZ30_18280)belonged to glycosyl family-5 and had interaction energy of−12.981 kcal/mol for the best docked cluster containing three out of ten docking conformations,and Tm value of 73.3℃.MD simulation of 100 ns proved highly stable binding interactions of CPZ30_18280 endoglucanase with cellulose with root mean square deviation(RMSD)values ranging from 0.15 to 0.30 nm.Consistent interactions with characteristic active site residues(tyrosine,tryptophan and aspartate)of glycosyl family-5 endoglucanases were found.Further,to enhance the production of endoglucanases,the fermentation conditions were optimized employing approaches like one factor at a time(OFAT)and response surface methodology(RSM).Maximum activity of endoglucanase was determined at 60℃.The optimized condi-tions for enhanced production of endoglucanase(10.15 U/mL)were pH 6.63,yeast extract conc.3.44 g/L,wheat bran 3.59%,and inoculum size 2.65%.Hence,P.lautus strain BHU3 has enormous potential to synthesize highly efficient thermostable endoglucanases under optimized regime using agro-wastes.Thus,it could find immense industrial applications including large scale cellulose conversion to bioethanol.

关 键 词：Agrowastes CELLULOSE RAST ENDOGLUCANASE Paenibacillus lautus 

分 类 号：O62[理学—有机化学]