Optimization of citrinin production by endophytic Penicillium citrinum isolated from Ziziphus jujuba  

作　　者：Hengrui Zhou Song Gao Weizhu Zeng Zhenshan Deng Jingwen Zhou 

机构地区：[1]Science Center for Future Foods,Jiangnan University,1800 Lihu Road,Wuxi 214122,Jiangsu,China [2]Key Laboratory of Industrial Biotechnology,Ministry of Education and School of Biotechnology,Jiangnan University,1800 Lihu Road,Wuxi 214122,Jiangsu,China [3]College of Life Sciences,Yan’an University,580 Shengdi Road,Yan’an 716000,Shanxi,China [4]Engineering Research Center of Ministry of Education On Food Synthetic Biotechnology,Jiangnan University,1800 Lihu Road,Wuxi 214122,Jiangsu,China

出　　处：《Systems Microbiology and Biomanufacturing》2022年第4期634-642,共9页系统微生物学与生物制造（英文）

基　　金：This work was supported by the National Key Research and Development Program of China(2018YFC1604102);the National Science Fund for Excellent Young Scholars(21822806).

摘　　要：Citrinin is a common mycotoxin produced by different fungi.Although considerable endeavors have been made to increase citrinin output to meet the requirements of research and industrial needs,few methods are effective,owing to potential safety concerns and technical limitations.In this study,a blue fungus that produces a yellow pigment was isolated from Ziziphus jujuba.The strain was then identified as Penicillium citrinum and named HR-087.Preparative liquid chromatography was used to extract the yellow pigment.Both LC-MS and NMR assays showed that it was citrinin.To enhance the citrinin pro-duction of this strain,the culture medium was optimized through orthogonal experiments,as well as batch and fed-batch process.The final titer and productivity of citrinin reached 9.62 g/L and 0.1 g/(L×h),respectively.The high titer achieved indicates the possibility of large-scale production of high purity citrinin for low-cost supply for both academic and diagnostic analysis standard.

关 键 词：MYCOTOXIN Penicillium citrinum Endophytic fungus Process optimization 

分 类 号：Q93[生物学—微生物学]