新冠肺炎感染者不同生物样本核酸和抗体检测结果分析  

Positivity of SARS-CoV-2 nucleic acid and antibody of different biological specimens from COVID-19 cases in Dongguan city——a comparative analysis

作　　者：徐霖 陈榕 洪韵 林春文 张泽武[2] 丁元林 钟庆杨 张巧利 于海兵 XU Lin;CHEN Rong;HONG Yun(Department of Epidemiology and Health Statistics,Guangdong Medical University,Dongguan,Guangdong Province 523808,China)

机构地区：[1]广东医科大学流行病与卫生统计学教研室,广东东莞523808 [2]东莞市疾病预防控制中心 [3]东莞市第九人民医院 [4]东莞市卫生健康局

出　　处：《中国公共卫生》2022年第8期1007-1010,共4页Chinese Journal of Public Health

基　　金：东莞市科技局项目(202071715044112);广东省教育厅专项(自然科学)(2020KZDZX1100);广东医科大学学科建设项目(4SG21276P)。

摘　　要：目的 探讨新冠肺炎患者不同生物标本核酸和抗体的结果变化特征。方法 于2020年1月23日—2020年10月9日,收集广东省东莞市101例新冠肺炎(COVID-19)确诊病例和35例无症状感染者各种生物样品,进行RT-PCR核酸检测和IgM、IgG抗体检测,并分析其结果。结果 新冠病毒(SARS-CoV-2)核酸阳性持续中位时间为7.00 d(0.00~27.00 d)。在COVID-19确诊患者发病0~7 d内,咽拭子核酸检测样本阳性率为48.21%(121/251),肛拭子核酸检测阳性率为36.00%(9/25),鼻咽拭子核酸检测阳性率为31.82%(7/22)。尿液样本在整个病程阶段均未检出新冠病毒样本核酸阳性。无症状感染者的咽拭子、肛拭子及鼻咽拭子核酸检测均有检出阳性,痰、粪便样本核酸检测未检出阳性。结论 随着新冠肺炎病程的不断推进,咽拭子、肛拭子及鼻咽拭子核酸阳性检出率随之降低,而血清中IgG抗体阳性检出率随之升高。Objective To investigate positive rate of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)nucleic acid and antibody of different biological samples from diagnosed coronavirus disease 2019(COVID-19) patients.Methods A variety of biological samples were collected from 101 confirmed COVID-19 cases and 35 asymptomatic infected persons in Dongguan city,Guangdong province from January 23 to October 9,2020.SARS-CoV-2 nucleic acid was detected with real-time fluorescence quantitative reverse transcriptase polymerase chain reaction(RT-PCR);serum immunoglobulin M(IgM) and IgG antibodies against SARS-CoV-2 were measured with colloidal gold method.The detection results were analyzed statistically.Results The median duration of persistent positivity of SARS-CoV-2 nucleic acid the was 7.00 days(interquartile range:0.00 – 27.00 days) for all the patients.Within 0 – 7 days after the onset of COVID-19,the positive rates were 48.21%(121/251),36.00%(9/25),and 31.82%(7/22) for pharyngeal,anal,and nasopharyngeal swab specimens,respectively.No SARS-CoV-2 nucleic acid positive urine samples were detected during whole course of the disease for all the patients.SARS-CoV-2 nucleic acid positive pharyngeal,anal,and nasopharyngeal swab specimens were detected but no positive sputum and feces samples were detected among the asymptomatic patients.Conclusion With the progress of disease,the positive rate of SARS-CoV-2 nucleic acid decreases for pharyngeal,anal,and nasopharyngeal swab specimens from COVID-19 patients but that of serum IgG antibody against SARS-CoV-2 increases.

关 键 词：新冠肺炎 SARS-CoV-2 生物样本 

分 类 号：R563.1[医药卫生—呼吸系统]