炎症标志物在砷致大鼠肺损伤中表达及意义  

Expression and significance of inflammatory markers in rats with arsenic-induced lung injury

作　　者：王文娟[1] 郑凡艳 王庆陵[1] 张爱华[1] WANG Wen-juan;ZHENG Fan-yan;WANG Qing-ling(Key Laboratory of Ministry of Education for Environmental Pollution Monitoring and Disease Control,Department of Toxicology,School of Public Health,Guizhou Medical University,Guiyang,Guizhou Province 550025,China)

机构地区：[1]贵州医科大学环境污染与疾病监控教育部重点实验室/公共卫生学院毒理学系,贵阳550025

出　　处：《中国公共卫生》2022年第8期1039-1042,共4页Chinese Journal of Public Health

基　　金：国家自然科学基金(81872569,U1812403)。

摘　　要：目的探讨亚砷酸钠(NaAsO_(2))染毒大鼠肺损伤效应及炎症标志物表达情况及意义。方法32只Wistar大鼠,按体重随机分为4组:对照组、2.5、5和10 mg/kg/d染毒剂量组,连续灌胃4个月。免疫荧光法和ELISA法分别检测Clara细胞蛋白(CC16)、基质金属蛋白酶–9(MMP-9)和金属蛋白酶组织抑制物–1(TIMP-1)在肺组织和血清中的表达水平,组织和血清中蛋白表达的相关性采用Pearson相关性分析。结果自实验90 d开始,中、高砷剂量组大鼠体重降低(P<0.05);NaAsO_(2)染毒组大鼠肺组织和血清中CC16表达水平均降低(P<0.05),肺组织CC16表达水平与其在血清中的含量呈正相关(r=0.89,P<0.05);NaAsO_(2)染毒组大鼠肺组织和血清中MMP-9表达水平均升高(P<0.05),肺组织MMP-9表达水平与其在血清中的含量呈正相关(r=0.50,P<0.05);MMP-9/TIMP-1在肺组织中的表达与血清中水平呈正相关(r=0.38,P<0.05)。结论砷暴露诱导了大鼠肺组织损伤,炎症标志物与砷致大鼠肺损伤发生发展有关。Objective To investigate lung injury effect of sodium arsenite(NaAsO_(2))in rats and the expression and significance of inflammatory markers associated with the injury.Methods Thirty-two Wistar rats were randomly divided into four groups according to body weight:a control group and three groups with 4-month gavage administration of NaAsO_(2) at dosages of 2.5,5,and 10 mg/kg per day.The expression of Clara cell secretory protein(CC16),matrix metalloproteinase-9(MMP-9)and tissue inhibitor of metalloproteinases-1(TIMP-1)in lung tissue and serum were detected with immunofluorescence assay and enzyme-linked immunosorbent assay(ELISA).Pearson correlation analysis was adopted to assess the association between expressions of the markers in lung tissues and serum.Results After 90 days′treatment,the body weight of the rats exposed to moderate-and high-dose NaAsO_(2) decreased significantly(P<0.05).For the rats with NaAsO_(2) treatment,the expression of CC16 in lung tissue and serum of reduced significantly(P<0.05)and the expression of CC16 in lung tissue was positively correlated with that in serum(r=0.89,P<0.05);while,the expression of MMP-9 in lung tissue and serum increased significantly(P<0.05)and MMP-9 expression in lung tissue was positively correlated with that in serum(r=0.50,P<0.05);the ratio of MMP-9/TIMP-1 expression in lung tissue was positively correlated with the ratio of the expressions in serum(r=0.38,P<0.05).Conclusion Arsenic exposure can induce lung injury in rats and expressions of inflammatory markers in lung tissue and serum are associated with the development of arsenic-induced lung injury.

关 键 词：砷 肺 炎症 组织 血清 

分 类 号：R99[医药卫生—毒理学]