CRISPR/Cas9-Targeted Knockout of Rice Susceptibility Genes OsDjA2 and OsERF104 Reveals Alternative Sources of Resistance to Pyricularia oryzae  

作　　者：Fabiano T.P.KTÁVORA Anne Cécile MEUNIER Aurore VERNET Murielle PORTEFAIX Joëlle MILAZZO Henri ADREIT Didier THARREAU Octávio LFRANCO Angela MEHTA 

机构地区：[1]Department of Genetics and Biotechnology,Federal University of Juiz de Fora,Juiz de Fora-MG 36036330,Brazil [2]Embrapa Genetic Resources and Biotechnology,Brasília-DF 70770-917,Brazil [3]Genetic Improvement and Adaptation of Mediterranean and Tropical Plants,Agricultural Research Center for International Development(CIRAD),Montpellier 34398 CEDEX 5,France [4]CIRAD-INRAe-Agro Institute,University of Montpellier,Montpellier 34000,France [5]Plant Health Institute of Montpellier,CIRAD,Montpellier 34398,France [6]University of Montpellier,National Institute of Agronomic Research,Research Institute for Development,Montpellier SupAgro,Montpellier 34398,France [7]Department of Genomics Science and Biotechnology,Proteomics Analysis Center,Catholic University of Brasília,Brasília-DF 71966-900,Brazil [8]S-Inova Biotech,Catholic University of Dom Bosco,Campo Grande-MS 79117-900,Brazil

出　　处：《Rice science》2022年第6期535-544,I0023-I0030,共18页水稻科学（英文版）

基　　金：financially supported by Brazilian Agricultural Research Corporation (Embrapa)-Coordination for the Improvement of Higher Education Personnel;National Council for Scientific and Technological Development, Federal District Research Support Foundation;Foundation for Scientific and Technological Development of Mato Grosso do Sul State

摘　　要：Rice genes OsDjA2 and OsERF104,encoding a chaperone protein and an APETELA2/ethylene-responsive factor,respectively,are strongly induced in a compatible interaction with blast fungus,and also have function in plant susceptibility validated through gene silencing.Here,we reported the CRISPR/Cas9 knockout of OsDjA2 and OsERF104 genes resulting in considerable improvement of blast resistance.A total of 15 OsDjA2(62.5%)and 17 OsERF104(70.8%)T_(0)transformed lines were identified from 24 regenerated plants for each target and used in downstream experiments.Phenotyping of homozygous T1 mutant lines revealed not only a significant decrease in the number of blast lesions but also a reduction in the percentage of diseased leaf area,compared with the infected control plants.Our results supported CRISPR/Cas9-mediated target mutation in rice susceptibility genes as a potential and alternative breeding strategy for building resistance to blast disease.

关 键 词：gene editing plant-pathogen interaction Magnaporthe pathosystem plant immunity blast resistance S-gene RICE 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]