祛风止痛胶囊HPLC指纹图谱建立及7种成分同时测定  

Establishment of HPLC fingerprints for Qufeng Zhitong Capsules and simultaneous determination of seven constituents

作　　者：廖禹程 丁一 张娟利 王婧雯 文爱东 李锐莉 胡君萍[1] 杨建华[1,3] LIAO Yu-cheng;DING Yi;ZHANG Juan-li;WANG Jing-wen;WEN Ai-dong;LI Rui-li;HU Jun-ping;YANG Jian-hua(College of Pharmacy,Xinjiang Medical University,Urumqi 830011,China;Department of Pharmacy,The First Affiliated Hospital of Air Force Military Medical University,Xi’an 710032,China;The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830011,China)

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830011 [2]空军军医大学第一附属医院药剂科,陕西西安710032 [3]新疆医科大学第一附属医院,新疆乌鲁木齐830011

出　　处：《中成药》2022年第10期3111-3114,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(82074321);新疆天然药物活性组分与释药技术重点实验室项目(XJDX1713);陕西省重点研发计划社会发展项目(2021SF-379)。

摘　　要：目的 建立祛风止痛胶囊(老鹳草、槲寄生、续断等)HPLC指纹图谱,并同时测定没食子酸、马钱苷酸、紫丁香苷、柯里拉京、马钱苷、鞣花酸、蛇床子素含量。方法 该药物甲醇提取液的分析采用Agilent C色谱柱(250 mm×4.6 mm, 5μm);流动相甲醇-0.2%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温35℃;检测波长254 nm。结果 10批样品HPLC指纹图谱中有21个共有峰,相似度均大于0.986。7种成分在各自范围内线性关系良好(r≥0.999 7),平均加样回收率99.1%~101.5%,RSD 1.42%~1.96%。结论 该方法简便高效,稳定可靠,可用于祛风止痛胶囊的质量控制。AIM To establish the HPLC fingerprints for Qufeng Zhitong Capsules(Geranii Herba, Visci Herba, Dipsaci Radix, etc.) and to simultaneously determine the contents of gallic acid, loganylic acid, syringin, corilagin, loganin, ellagic acid and osthole.METHODS The analysis of methanol extract of this drug was performed on a 35 ℃ thermostatic Agilent Ccolumn(250 mm×4.6 mm, 5 μm), with the mobile phase comprising of methanol-0.2% phosphoric acid flowing at 1.0 mL/min in a gradient elution manner, and the detection wavelength was set at 254 nm.RESULTS There were twenty-one common peaks in the HPLC fingerprints of ten batches of samples with the similarities of more than 0.986. Seven constituents showed good linear relationships within their own ranges(r≥0.999 7), whose average recoveries were 99.1%-101.5% with the RSDs of 1.42%-1.96%.CONCLUSION This simple, efficient, stable and reliable method can be used for the quality control of Qufeng Zhitong Capsules.

关 键 词：祛风止痛胶囊 HPLC指纹图谱 化学成分 

分 类 号：R927.2[医药卫生—药学]