温清饮HPLC指纹图谱建立及7种成分同时测定  

Establishment of HPLC fingerprints for Wenqingyin and simultaneous determination of seven constituents

作　　者：司徒惠君 王谊 王宓 唐铭蔚 任孟月 SITU Hui-jun;WANG Yi;WANG Mi;TANG Ming-wei;REN Meng-yue(College of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China)

机构地区：[1]广东药科大学中药学院,广东广州510006

出　　处：《中成药》2022年第10期3115-3119,共5页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(82004252);广州市科学技术局基础与应用基础研究一般项目(2060206)。

摘　　要：目的 建立温清饮(当归、川芎、白芍等)HPLC指纹图谱,并同时测定没食子酸、药根碱、黄芩苷、小檗碱、汉黄芩苷、黄芩素、汉黄芩素的含量。方法 该药物甲醇提取液的分析采用Agilent Polaris-C色谱柱(250 mm×4.6 mm, 5μm);流动相0.2%磷酸-乙腈,梯度洗脱;体积流量0.8 mL/min;柱温30℃;检测波长270 nm。结果 10批样品HPLC指纹图谱中有30个共有峰,相似度大于0.963。7种成分在各自范围内线性关系良好(r>0.990 0),平均加样回收率96.99%~100.17%,RSD 1.05%~1.85%。结论 该方法稳定可靠,可用于温清饮的质量控制。AIM To establish the HPLC fingerprints for Wenqingyin(Angelicae sinensis Radix, Chuanxiong Rhizoma, Paeoniae Radix Alba, etc.) and to simultaneously determine the contents of gallic acid, jatrorrhizine, baicalin, berberine, baicalein, wogonoside and wogonin.METHODS The analysis of methanol extract of this drug was performed on a 30 ℃ thermostatic Agilent Polaris-Ccolumn(250 mm×4.6 mm, 5 μm), with the mobile phase comprising of 0.2%phosphoric acid-acetonitrile flowing at 0.8 mL/min in a gradient elution manner, and the detection wavelength was set at 270 nm.RESULTS There were thirty common peaks in the fingerprints of ten batches of samples with the similarities of more than 0.963. Seven constituents showed good linear relationships within their own ranges(r>0.990 0), whose average recoveries were 96.99%-100.17%with the RSDs of 1.05%-1.85%.CONCLUSION This stable and reliable method can be used for the quality control of Wenqingyin.

关 键 词：温清饮 HPLC指纹图谱 化学成分 

分 类 号：R927.2[医药卫生—药学]