HAp增强PLA纤维基人工韧带的骨细胞活性研究  

Study on osteocyte activity of HAp enhanced PLA fiber-based artificial ligament

作　　者：陆腱 李宏 刘桂连 吴琪琳[1] 华英汇 费翔 朱美芳[1] LU Jian;LI Hong;LIU Guilian;WU Qilin;HUA Yinghui;FEI Xiang;ZHU Meifang(College of Materials Science and Engineering,State Key Laboratory for Modification of Chemical Fibersand Polymer Materials,Donghua University,Shanghai 201600;Department of Sports Medicine,Huashan Hospital Affiliated to Fudan University,Shanghai 200040)

机构地区：[1]东华大学材料科学与工程学院纤维材料改性国家重点实验室,上海201600 [2]复旦大学附属华山医院运动医学科,上海200040

出　　处：《合成纤维工业》2022年第5期11-16,共6页China Synthetic Fiber Industry

摘　　要：基于聚乳酸(PLA)纤维的熔融纺丝技术,在PLA纤维内有效杂化引入促进骨修复的纳米羟基磷灰石(HAp)功能粒子,制备PLA-HAp杂化纤维;参照蚕丝基人工韧带编织工艺,制备PLA-HAp杂化纤维基人工韧带;从形貌、结构、力学性能、细胞相容性方面研究了HAp的引入对PLA-HAp杂化纤维及其人工韧带的影响。结果表明:HAp的加入可提高PLA纤维的结晶度和取向度,但其与PLA的相容性较差;加入HAp质量分数0.5%~1.5%,可以获得断裂强度达3.16~3.27 cN/dtex的PLA-HAp杂化纤维;将PLA-HAp杂化纤维编织成人工韧带,PLA-HAp纤维基人工韧带的拉伸强度达87.1~94.2 MPa;HAp的加入可有效在人工韧带表面引入钙、磷元素,从而提升人工韧带与骨细胞(MC3T3)的相容性;通过与骨细胞(MC3T3)的体外共培养实验,发现负载HAp可有效提高PLA-HAp杂化纤维基人工韧带表面骨细胞活性。Based on the melt spinning technology of polylactic acid(PLA)fibers,nano hydroxyapatite(HAp)functional particles to promote bone repair were effectively hybridized into PLA fibers to prepare PLA-HAp hybrid fibers.PLA-HAp hybrid fiber-based artificial ligament was prepared according to the weaving process of silk based artificial ligament.The effects of the introduction of HAp on PLA-HAp hybrid fibers and artificial ligaments thereof were studied from the aspects of morphology,structure,mechanical properties and cell compatibility.The results showed that the addition of HAp could improve the crystallinity and orientation degree of PLA fiber,but its compatibility with PLA was poor;PLA-HAp hybrid fibers with a breaking strength of 3.16-3.27 cN/dtex can be obtained by adding 0.5%-1.5%HAp by mass fraction;PLA-HAp hybrid fibers were woven into artificial ligament,which had the tensile strength of 87.1-94.2 MPa;and the addition of HAp can effectively introduce calcium and phosphorus on the surface of artificial ligament,thus improving the compatibility between artificial ligament and osteocyte cells(MC3T3);and it was found through co-culture of bone cells(MC3T3)in vitro that HAp loading could effectively improve the activity of osteocyte on the surface of PLA-HAp hybrid fiber-based artificial ligament.

关 键 词：聚乳酸纤维 羟基磷灰石 杂化纤维 人工韧带 骨细胞活性 

分 类 号：TQ342.87[化学工程—化纤工业]