农华麻鸭脑组织多胺含量及其代谢相关基因表达的研究  

Study on Polyamine Content and Expression of Polyamine Metabolism Genes in Brain Tissues from Nonghua Ducks(Anas platyrhynchos)

作　　者：康丽鹃 周雪敏 但红颖 葛凡滋 李硕 王鑫 王泽龙 牛春阳 郭永妮 康波[1] KANG Li-Juan;ZHOU Xue-Min;DAN Hong-Ying;GE Fan-Zi;LI Shuo;WANG Xin;WANG Ze-Long;NIU Chun-Yang;GUO Yong-Ni;KANG Bo(College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611100,China)

机构地区：[1]四川农业大学动物科技学院,成都611100

出　　处：《农业生物技术学报》2022年第10期1954-1961,共8页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31872358;32172727)。

摘　　要：多胺主要包括腐胺、亚精胺和精胺,可参与调控神经系统发育和再生。为研究鸭(Anas platyrhynchos)脑组织多胺代谢发育性变化规律,本研究采用高效液相色谱技术,测定0~120日龄的农华麻鸭大脑和小脑组织多胺含量,应用qPCR检测多胺代谢关键基因表达量。结果表明,大脑和小脑中亚精胺和精胺含量均高于腐胺,且随着日龄增长多胺总量呈下降趋势。在大脑中,60日龄腐胺含量(30.80 mg/kg)显著高于其他日龄(P<0.05),0日龄亚精胺含量(643.26 mg/kg)显著高于其他日龄(P<0.05),0日龄精胺含量(1665.95 mg/kg)和120日龄精胺含量(1623.66 mg/kg)显著高于其他日龄(P<0.05)。在小脑中,0日龄亚精胺含量(54.09 mg/kg)和精胺含量(413.50 mg/kg)显著高于其他日龄(P<0.05),0日龄腐胺含量(26.46 mg/kg)和120日龄腐胺含量(22.03 mg/kg)显著高于其他日龄(P<0.05)。大脑和小脑中多胺代谢基因均在0日龄时高表达(P<0.05)。大脑多胺代谢关键基因表达量均呈30日龄时下降,60日龄升高,90日龄下降,120日龄上升的趋势。小脑中,各基因表达水平在0日龄时表达量均处于较高水平,表达量变化趋势不一致。本研究揭示了不同日龄的鸭脑组织中多胺含量变化规律及多胺合成代谢关键基因表达水平,为多胺调控脑生长发育提供基础数据。Polyamines are involved in regulating the development and regeneration of nervous system.To study the developmental changes of polyamine metabolism in cerebrum and cerebellum tissues from Nonghua ducks(Anas platyrhynchos)at different day-age,polyamine content in cerebrum and cerebellum tissues from ducks at 0~120 day-age were determined by HPLC.The relative abundance of genes related to polyamine metabolism were determined by qPCR.The result showed that contents of spermidine and spermine in cerebrum and cerebellum tissues were significantly higher than putrescine(P<0.05),and the contents of polyamine decreased with the increase of day-age.Putrescine content(30.80 mg/kg)in cerebrum tissues at 60day-age was significantly higher than others(P<0.05).Spermidine content(643.26 mg/kg)in cerebrum tissues at 0 day-age was significantly higher than others(P<0.05).Spermine content in cerebrum tissues at 0day-age(1665.95 mg/kg)and 120 day-age(1623.66 mg/kg)were significantly higher than others(P<0.05).Spermidine(54.09 mg/kg)and spermine(413.5 mg/kg)contents in cerebellum tissues at 0 day-age were significantly higher than others(P<0.05).Putrescine content in cerebellum tissues at 0 day-age(26.46 mg/kg)and 120 day-age(22.03 mg/kg)were significantly higher than others(P<0.05).The expression levels of key genes related to polyamine metabolism decreased in cerebrum tissues of ducks at 30 day-age,increased at 60day-age,decreased at 90 day-age,and then increased at 120 day-age.The expression levels of key genes related to polyamine metabolism in cerebellum tissues of ducks at 0 day-age were relatively higher than that of other ducks.The trend in gene expression levels was different.This study reveals the changes of polyamine content and the expression levels of key genes related to polyamine metabolism in brain tissues from different age ducks,and provides basic data for polyamine regulating brain growth and development in ducks.

关 键 词：多胺 多胺代谢 基因表达 大脑 小脑 

分 类 号：S835.5[农业科学—畜牧学]