106株红色毛癣菌基因分型与药敏分析  

The genotyping of Trichophyton rubrum and the analysis of drug sensitivity

作　　者：郑文爱 乔凤 唐小正 吴伟伟 王皇玉 ZHENG Wenai;QIAO Feng;TANG Xiaozheng;WU Weiwei;WANG Huangyu(Department of Clinical Laboratory,the Fifth People’s Hospital of Haina province,Haikou 570206,China;Department of Dermatology,the Fifth People’s Hospital of Haina province,Haikou 570206,China)

机构地区：[1]海南省第五人民医院检验科,海口570206 [2]海南省第五人民医院皮肤科,海口570206

出　　处：《中国真菌学杂志》2022年第5期363-367,共5页Chinese Journal of Mycology

基　　金：海南省自然科学基金面上项目(819MS132);海南省卫生计生行业科研项目(18A200003)。

摘　　要：目的探索海南地区的红色毛癣菌基因型与感染部位、药敏的关系。方法基于红色毛癣菌核糖体rDNA非转录区(NTS)的基因分型进行种内分型,依据“CLSI-M38-A2”方案进行药敏实验。结果106株红色毛癣菌TRS-1基因型有5种带型,其中TypeⅠ52株(49.06%),TypeⅡ14株(13.21%),TypeⅢ5株(4.72%),TypeⅣ型3株(2.83%),其他带型32株(30.18%)。TRS-2基因型有3种带型,其中TypeⅠ68株(64.15%),TypeⅡ6株(5.66%),其他带型32株(30.19%)。药敏结果MIC几何均数由低至高分别为特比萘芬(0.0092μg/mL)、伏立康唑(0.0181μg/mL)、伊曲康唑(0.1491μg/mL)、酮康唑(0.1630μg/mL)、氟康唑(2.3164μg/mL)。有42株菌表现出对抗真菌药物不敏感,不敏感菌株TRS-1和TRS-2分型均以TypeⅠ为主。结论海南地区流行的红色毛癣菌TRS-1和TRS-2基因型均以TypeⅠ为主。以对特比萘芬(0.0092μg/mL)、伏立康唑(0.0181μg/mL)MIC几何均数最低。本结果发现本地区的红色毛癣菌不敏感菌株与基因带型关系不大,也与感染部位无关,可能与来源有关。Objective To study the relationship between the genotype of Trichophyton rubrum and the site of infection and drug sensitivity in the area.Methods Perform intraspecies typing based on the genotyping of the ribosomal rDNA non-transcribed region(NTS)of Trichophyton rubrum,and test the drug sensitivity according to the"CLSI-M38-A2"protocol.Results There were five banding types in 106 Trichophyton rubrum TRS-1 genotypes,including TypeⅠ52(49.06%),TypeⅡ14(13.21%),TypeⅢ5(4.72%),TypeⅣ3(2.83%),and others 32 strains(30.18%).There are three banding types in TRS-2 genotypes,including TypeⅠ68 strains(64.15%),TypeⅡ6 strains(5.66%),and others 32 strains(30.19%).The geometric mean MICs of drug sensitivity results from low to high were terbinafine(0.0092μg/mL),voriconazole(0.0181μg/m),itraconazole(0.1491μg/mL),ketoconazole(0.1630μg/mL),and fluconazole(2.3164μg/mL).42 strains showed insensitivity to antifungal drugs,and the insensitive strains TRS-1 and TRS-2 were mainly type I.Conclusion Both the TRS-1 and TRS-2 genotypes of Trichophyton rubrum in Hainan are mainly TypeⅠ.Terbinafine(0.0092μg/mL)and voriconazole(0.0181μg/mL)have the lowest geometric mean MICs.This result found that the insensitive strains in this area have little relationship with the red hair genotype,and have nothing to do with the infection site,and may be related to the source.

关 键 词：红色毛癣菌 基因型 TRS 药敏试验 

分 类 号：R379.2[医药卫生—病原生物学]