饲粮中添加柠檬酸对黄羽肉鸡生长性能、肠道免疫功能和菌群结构的影响  

Effects of dietary supplementation with citric acid on growth performance,intestinal immunity function and microflora structure of yellow-feathered broilers

作　　者：林厦菁 茅沈丽 叶金玲 苟钟勇 王一冰 范秋丽 蒋守群 LIN Xiajing;MAO Shenli;YE Jinling;GOU Zhongyong;WANG Yibing;FAN Qiuli;JIANG Shouqun(Institute of Animal Science,Guangdong Academy of Agricultural Sciences,State Key Laboratory of Livestock and Poultry Breeding,Key Laboratory of Animal Nutrition and Feed Science in South China,Ministry of Agriculture and Rural Affairs,Guangdong Public Laboratory of Animal Breeding and Nutrition,Guangdong Key Laboratory of Animal Breeding and Nutrition,Guangzhou Guangdong 510640)

机构地区：[1]广东省农业科学院动物科学研究所,畜禽育种国家重点实验室,农业部农村部华南动物营养与饲料重点实验室,广东省畜禽育种与营养研究重点实验室,广东广州510640

出　　处：《广东畜牧兽医科技》2022年第5期1-5,58,共6页Guangdong Journal of Animal and Veterinary Science

基　　金：国家现代农业产业技术体系(CARS-41-G10);广东省重点领域研发计划项目(2020B0202090004);国家重点研发专项(2021YFD300404);国家自然科学基金青年基金(31802104);广东省自然科学基金项目(2021A1515012412、2021A1515010830);广东省农业科学院科技计划项目(202106TD、R2019PY-QF008)。

摘　　要：本试验旨在研究柠檬酸对黄羽肉鸡生长性能、免疫功能的影响。选用320只1日龄健康发育良好的快速型麻黄鸡(公、母各半),按照体重一致原则随机分为2个处理组,每处理8个重复,每重复20只鸡,基础饲粮中分别添加0、120 mg/kg柠檬酸。试验鸡自由采食和饮水,试验期共21天。试验结果显示,空白对照组和柠檬酸组试验鸡日增重、采食量和料重比均无显著差异(P>0.05);与对照组相比,柠檬酸组日增重提高0.52%,采食量提高1.57%,料重比提高0.60%。饲粮中添加柠檬酸对黄羽肉鸡的心脏、肝脏、脾脏、肺脏、肾脏指数无显著影响(P>0.05);与对照组相比,柠檬酸组的心脏指数提高1.56%,肝脏指数提高25%,肺脏指数提高0.98%,肾脏指数降低3.28%。饲粮中添加柠檬酸对试验鸡空肠粘膜的免疫因子IL-6、IL-8、IL-10、IL-1β、IFN-γ、TNF-α、sIgA的含量均无显著影响(P>0.05);与对照组相比,柠檬酸组的空肠黏膜免疫因子IL-1β、IL-6、IL-8、IL-10、IFN-γ、sIgA、TNF-α的含量分别提高17.54%、10.95%、3.30%、4.59%、0.77%、28.35%、10.48%。饲粮中添加柠檬酸会显著降低试验鸡空肠的绒毛高度和隐窝深度(P<0.05),但对空肠的绒隐比无显著影响(P>0.05);显著降低回肠绒毛高度(P<0.05),绒隐比比对照组高14.7%(P>0.05)。添加柠檬酸对试验鸡盲肠微生物菌群组成无显著影响(P>0.05),与对照组相比,柠檬酸组盲肠中大肠杆菌、沙门氏菌、乳酸杆菌和双歧杆菌的水平分别提高0.91%、4.14%、0.14%、0.84%;总菌数降低0.19%。综合以上所有试验结果说明黄羽肉仔鸡饲粮中添加120mg/kg的柠檬酸,对其生长性能、肠道细胞因子含量和盲肠菌群结构均无显著影响,对回肠黏膜形态结构有一定改善作用。This experiment was conducted to investigate the effects of citric acid on growth performance and immune function of yellow-feathered broilerse. A total of three hundred and twenty 1-day-old yellow-feathered broilers(male and female half)were randomly divided into 2 groups with 8 replicate of 20 birds per replicate. The basal diets were supplemented with 0 and 120 mg/kg citric acid,respectively. The experiment lasted for 21 days. The results showed that dietary citric acid supplementation had no significant effect on the ADG,ADFI,and F/G(P>0.05)compared with the control group,the ADG of citric acid group increased by 0.52%,ADFI increased by 1.57%;F/G increased by 0.60%. Citric acid had no significant effects on the heart,liver,spleen,lung,and kidney indexes of yellow-feathered broilers at 21 days of age(P>0.05)compared with the control group,the heart index of the citric acid group increased by 1.56%,liver index increased by 25%,lung index increased by 0.98%,renal index decreased by3.28%. Compared with the control group,dietary supplementation with citric acid had no significant effects on the contents of IL-6,IL-8,IL-10,IL-1β,IFN-γ,TNF-α and sIgA in jejunum mucosa(P>0.05),the contents of jejunum mucosal immune factors IL-1β、IL-6、IL-8、IL-10、IFN-γ、sIgA and TNF-α in citric acid group were increased by17.54%,10.95%,3.30%,4.59%,0.77%,28.35% and 10.48%,respectively. Dietary supplementation with citric acid significantly decreased the villus height and crypt depth of jejunum(P<0.05),while had no significant effect on the villus height to crypt depth ratio of jejunum(P>0.05). The ileum villus height was significantly decreased(P<0.05),but the villus height to crypt depth ratio was 14.7% higher than that of the control group(P>0.05). Dietary supplementation with citric acid had no significant effect on cecalmicroflora composition(P>0.05)compared with the control group,the number of Escherichia coli,Salmonella,Lactobacillus and Bifidobacteria in cecum of citric acid group were increased by 0.91%,4.14%,0.14

关 键 词：柠檬酸 黄羽肉鸡 肠道形态结构 细胞因子 

分 类 号：S831.5[农业科学—畜牧学]