Cellular responses to nanoscale substrate topography of TiO_(2) nanotube arrays: cell morphology and adhesion  

作　　者：Monchupa Kingsak Panita Maturavongsadit Hong Jiang Qian Wang 

机构地区：[1]Department of Chemistry and Biochemistry,University of South Carolina,Columbia,SC,USA [2]Computer Science,Physics,and Engineering Department,Benedict College,Columbia,SC,USA

出　　处：《Biomaterials Translational》2022年第3期221-233,共13页生物材料转化电子杂志（英文）

基　　金：support The authors gratefully acknowledge financial support from the National Science Foundation and SC EPSCoR IDeA program under NSF Award#OIA 1655740;GEAR CRP Award Number 18-GC01.

摘　　要：Nanotopographical features can be beneficial in augmenting cell functions and increasing osteogenic potential.However,the relationships between surface topographies and biological responses are difficult to establish due to the difficulty in controlling the surface topographical features at a low-nanometre scale.Herein,we report the fabrication of well-defined controllable titanium dioxide(TiO_(2))nanotube arrays with a wide range of pore sizes,30-175 nm in diameter,and use of the electrochemical anodization method to assess the effect of surface nanotopographies on cell morphology and adhesion.The results show that TiO_(2) nanotube arrays with pore sizes of 30 and 80 nm allowed for cell spreading of bone marrow-derived mesenchymal stem cells with increased cell area coverage.Additionally,cell adhesion was significantly enhanced by controlled nanotopographies of TiO_(2) nanotube arrays with 80 nm pore size.Our results demonstrate that surface modification at the nano-scale level with size tunability under controlled chemical/physical properties and culture conditions can greatly impact cell responses.These findings point to a new direction of material design for bone-tissue engineering in orthopaedic applications.

关 键 词：cell adhesion cellular responses morphology NANOTOPOGRAPHY TiO_(2)nanotube arrays 

分 类 号：TB383[一般工业技术—材料科学与工程]