Molecular characterization,expression patterns,and subcellular localization of a classical and a novel nonclassical MHC class Iαmolecules from Japanese eel Anguilla japonica  

作　　者：Jianjun Feng Xinwei Peng Peng Lin Yilei Wang Ziping Zhang Yuankai Xu Pengyun Chen Jingying Wu 

机构地区：[1]Engineer Research Center of Eel Modern Industry Technology,Ministry of Education,Xiamen,361021,China [2]College of Fisheries,Jimei University,Xiamen,361021,China [3]College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou,350002,China [4]Ningbo Institute of Oceanography,Ningbo,315832,China

出　　处：《Aquaculture and Fisheries》2023年第2期108-123,共16页渔业学报（英文）

基　　金：supported by“Nature Science Foundation of Fujian Province”(No.2020J01671);“The Science Foundation of Jimei University,China”(No.ZP2020017);“Engineering Research Center of the Modern Technology for Eel Industry,Ministry of Education,P.R.China”(No.RE202110);“Key Laboratory and Healthy Mariculture for the East China Sea,Ministry of Agriculture and Rural Affairs,P.R.China”(No.2020ESHML02).

摘　　要：Major histocompatibility complex(MHC)molecules play a critical role in the immune response of vertebrate animals by presenting foreign antigens to T lymphocytes.In this study,we first cloned and identified a classical and a novel nonclassical MHC Iαmolecules from Japanese eel(Anguilla japonica),named as AjMHC I-UBA and AjMHC I-L,respectively.The full-length cDNA of AjMHC I-UBA contains an open reading frame(ORF)of 1047 bp encoding a predicted protein of 348 amino acids,while AjMHC I-L 1089 bp encodes 362 amino acids.The multiple alignment of the amino acid sequence showed that AjMHC I-UBA and AjMHC I-L consist of an N-terminal MHC I superfamily domain withinα1 andα2 helices,an IgC-MHC Iα3 domain,and a transmembrane region close to the C-terminal,which are similar to other fish and mammal species.Molecular polymorphism analysis showed that eight different major alleles of AjMHC I-UBA,named as AjMHC I-UBA*0101~1001,were identified from six Japanese eel individuals.Furthermore,a distinguishing signature of the nonclassical L-lineage genes specific motif“HINMTL”,including an N-glycosylation site(NXS/T),was present in theα3 domain of AjMHC I-L sequences.Although the predicted three-dimensional structures of AjMHC I-UBA and AjMHC I-L are similar to that of human MHC Iα,phylogenetic analysis showed that these two protein molecules belong to classical MHC I UBA gene of U-lineage and nonclassical MHC I gene of L-lineage,respectively.Quantitative real-time polymerase chain reaction(qRT-PCR)analysis revealed that the highest expression of AjMHC I-UBA and AjMHC I-L was found in the intestine and the expression level of AjMHC I-UBA in all of the examined tissues was significantly higher than that of AjMHC I-L.The expressions of AjMHC I-UBA and AjMHC I-L in liver,kidney and spleen were significantly induced following injection with the viral mimic poly I:C,LPS,and Aeromonas hydrophila infection.In vitro,the AjMHC I-UBA and AjMHC I-L transcripts of Japanese eel liver cells were significantly enhanced by the treatment

关 键 词：Anguilla japonica MHC Iα mRNA expression Subcelluar localization 

分 类 号：S917.4[农业科学—水产科学]