New prenylated flavonoid glycosides derived from Epimedium wushanense byβ-glucosidase hydrolysis and their testosterone production-promoting effects  

作　　者：SUN Xin-Guang PANG Xu LIANG Hai-Zhen ZHANG Jie WANG Bei LI Qi WANG Jie CHEN Xiao-Juan GUO Bao-Lin MA Bai-Ping 

机构地区：[1]Department of Pharmaceutical Sciences,Beijing Institute of Radiation Medicine,Beijing 100850,China [2]Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100193,China

出　　处：《Chinese Journal of Natural Medicines》2022年第9期712-720,共9页中国天然药物（英文版）

基　　金：supported by the National Science and Technology Major Project(No.2017ZX09301072);China Postdoctoral Science Foundation(No.2016M603041).

摘　　要：Six new prenylated flavonoid glycosides,including four new furan-flavonoid glycosides wushepimedoside A–D(1–4)and two new prenyl flavonoid derivatives wushepimedoside E–F(5–6),and one know analog epimedkoreside B(7)were isolated from biotransformation products of the aerial parts of Epimedium wushanense.Their structures were elucidated according to comprehensive analysis of HR-MS and NMR spectroscopic data,and the absolute configurations were assigned using experimental and calculated electronic circular dichroism(ECD)data.The regulatory activity of compounds 1–7 on the production of testosterone in primary rat Leydig cells were investigated,and 4 and 5 exhibited testosterone production-promoting activities.Molecular docking analysis suggested that bioactive compounds 4 and 5 showed the stable binding with 3β-HSD and 4 also had good affinity with Cyp17A1,which suggested that these compounds may regulate testosterone production through stimulating the expression of the above two key proteins.

关 键 词：Epimedium wushanense BIOTRANSFORMATION Prenylated flavonoids Regulation on testosterone production 

分 类 号：R284[医药卫生—中药学] R965[医药卫生—中医学]