HPLC法测定藏荆芥不同药用部位荆芥内酯的含量  

Determination of Nepetalactone in Different Medicinal Parts of Nepeta Angustifolia C.Y.Wu by HPLC

作　　者：杨阳 毛禹康 张琴 蔡鸿飞 李康强 张圳 许文东 Yang Yang;Mao Yukang;Zhang Qin;Cai Hongfei;Li Kangqiang;Zhang Zhen;Xu Wendong(Guangzhou Hanfang Pharmaceutical Co.,Ltd./National Engineering Research Center for Pharmaceutical Process Technology and New Drug Creation of Traditional Chinese Medicine,Guangzhou 510240,China)

机构地区：[1]广州白云山汉方现代药业有限公司/中药制药过程技术与新药创制国家工程研究中心,广东广州510240

出　　处：《广东化工》2022年第20期212-215,共4页Guangdong Chemical Industry

基　　金：国家科技部重点研发计划项目(2017YFC1703104);广州市科技计划项目(202002020009)。

摘　　要：目的:建立藏荆芥中荆芥内酯的含量测定方法。方法:采用高效液相色谱法,色谱柱选择:Supersil ODS2 250 mm×4.6 mm,5μm,流动相为乙腈-水(体积比为40∶60),流速为1.0 mL/min,检测波长为216 nm,柱温为30℃。结果:荆芥内酯的线性关系方程为Y=12795176.3884X+607348.6713,R^(2)=0.9994,在0.01~5.0 mg/mL范围内的峰面积与进样浓度线性关系良好(R^(2)=0.9994)。精密度、重复性及稳定性实验的相对标准偏差(RSD)分别为1.46%、1.19%、1.83%,平均加样回收率为97.66%,RSD为1.56%。藏荆芥中荆芥内酯的含量为0.86%,不同药用部位中荆芥内酯含量差异明显。结论:建立的高效液相色谱法方法简单、准确、快速,重复性好,专属性强,可应用于藏荆芥中荆芥内酯的含量测定。Objective: To establish a method for determination of Nepeta angustifolia C.Y.Wu. Methods: A high-performance liquid chromatography detection method was established. The column was Supersil ODS2 250 mm×4.6 mm, 5 μm and the mobile phase was acetonitrile-water(volume ratio 40∶60) at a flow rate of 1.0 mL/min. The detection wavelength was 216 nm, and the column temperature was 30 ℃. Results: The linear equation of nepetalactone was Y=12795176.3884X+607348.6713, and the peak area in the range of 0.01~5.0 mg/mL had a good linear relationship with the injection concentration(R^(2)=0.9994).The relative standard deviations(RSD) of precision, repeatability and stability experiments were 1.46 %, 1.19 % and 1.83 %, respectively. The average recovery rate was 97.66 % and RSD was 1.56 %. The content of nepetalactone in Nepeta angustifolia C.Y.Wu is 0.86 %, while which was obviously discrepant in different medicinal parts. Conclusion: The established HPLC method was simple, accurate, rapid, reproducible and specific, which could be applied to the determination of Nepeta angustifolia C.Y.Wu.

关 键 词：藏荆芥 荆芥内酯 含量测定 高效液相色谱 方法学 

分 类 号：TQ[化学工程]