手性固定相高效液相色谱法拆分右旋苯醚氰菊酯对映体  

Reslution of D-cyphenothrin Enaniomer s by HPLC with Chiral Stational Phase

作　　者：何永鹏 黄祖懿 He Yongpeng;Huang Zuyi(Institute of Chemical Engineering,Guangdong Academy of Sciences,Guangdong Key Laboratory of Industrial Surfactant,Guangzhou 510665,China)

机构地区：[1]广东省科学院化工研究所,广东省工业表面活性剂重点实验室,广东广州510665

出　　处：《广东化工》2022年第19期243-245,共3页Guangdong Chemical Industry

摘　　要：建立了以多糖衍生物为手性固定相的高效液相色谱直接拆分右旋苯醚氰菊酯的方法。在正相液相色谱条件下,考察了流动相中醇类改性剂的种类和浓度、流速、柱温对右旋苯醚氰菊酯4个立体异构体分离效果的影响,同时利用热力学方法对右旋苯醚氰菊酯的立体异构体与固定相保留和分离的热力学机理进行了探讨。结果表明,采用纤维素-三-(3,5-二甲基苯基氨基甲酸酯)手性色谱柱,在流动相为正己烷∶异丙醇∶二氯甲烷=98∶1∶1(V/V),流速1.0 mL/min,柱温25℃的条件下,可以实现右旋苯醚氰菊酯4个立体异构体的基线分离。A method for direct separation of d-cyphenothrin by high performance liquid chromatography(HPLC) using polysaccharide derivatives as chiral stationary phase have been established. The effects of alcohols, concentration, flow rate and column temperature on the separation of four stereoisomers of d-cyphenothrin in mobile phase are studied by reversed phase HPLC. The results indicate that when the mobile phase is n-hexane ∶ isopropyl ∶dichloromethane=98∶1∶1(V/V), flow rate with 1 mL/min, column temperature at 25 ℃, the cellulose tri(3,5-dimethylphenylcarbamate) chiral column could realize the baseline separation of four stereoisomers.

关 键 词：右旋苯醚氰菊酯 对映异构体 手性拆分 高效液相色谱 热力学机理 

分 类 号：TQ[化学工程]