碳硼烷基苯并咪唑鎓环蕃的合成及核苷酸识别性质研究  

Synthesis and Nucleotide Recognition Properties of Carborane-Based Benzoimidazolium Cyclophane

作　　者：林炳涵 卓继斌 林彩霞 高勇 袁耀锋[1] Lin Binghan;Zhuo Jibin;Lin Caixia;Gao Yong;Yuan Yaofeng(College of Chemistry,Fuzhou University,Fuzhou 350108;Fujian Metrology Institute,Fuzhou 350003;College of Chemistry and Materials Science,Fujian Normal University,Fuzhou 350117)

机构地区：[1]福州大学化学学院,福州350108 [2]福建省计量科学研究院,福州350003 [3]福建师范大学化学与材料科学学院,福州350117

出　　处：《有机化学》2022年第8期2551-2558,共8页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(Nos.21772023,22071025)资助项目。

摘　　要：以1,2-双(4-苄溴苯基)-邻碳硼烷为原料合成了两种新型的碳硼烷基苯并咪唑鎓类荧光探针4a和4b,通过IR,1H NMR,13C NMR,11B NMR和ESI-MS对其结构进行表征,并通过荧光、紫外光谱研究了探针4a和4b对核苷酸的识别性能.荧光滴定结果表明:探针4a和4b可以对单磷酸尿苷(UMP)实现on-off型响应,并具有良好的选择性和灵敏度,其中探针4b对于UMP的检测限可以达到3.41×10^(-8)mol/L.通过Job’s曲线和1HNMR滴定对探针4b的识别机理进行了初步的研究.Two novel carborane-based benzimidazolium fluorescent probes 4 a and 4 b were synthesized using 1,2-bis(4-benzylbromophenyl)-o-carborane as starting materials.The structures were characterized by IR,1 H NMR,13 C NMR,11 B NMR and ESI-MS,and the recognition properties of probes 4 a and 4 b for nucleotides were studied by fluorescence and ultraviolet spectroscopy.The results of fluorescence titration showed that probes 4 a and 4 b could achieve on-off response to uridine monophosphate(UMP),and had good selectivity and sensitivity.The detection limit of probe 4 b for UMP could reach 3.41×10^(-8)mol/L.The recognition mechanism of the probe 4 b was studied by Job’s curve and 1 H NMR titration.

关 键 词：碳硼烷 苯并咪唑 识别 荧光淬灭 单磷酸尿苷 

分 类 号：O657.3[理学—分析化学]