3.0T MR T1ρ及T2 mapping评估兔股骨内侧髁关节软骨退变  

3.0T MR T1ρand T2 mapping for evaluating articular cartilage degeneration of medial femoral condyle in rabbits

作　　者：罗慕晴 李宏伟 向辉春 何业文 刘音其 黎建宇 颜路悠 张堃[1] 李平[1] LUO Muqing;LI Hongwei;XIANG Huichun;HE Yewen;LIU Yinqi;LI Jianyu;YAN Luyou;ZHANG Kun;LI Ping(Department of Radiology,the First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China;Department of Radiology,Huaihua Hospital of Traditional Chinese Medicine,Huaihua 418099,China)

机构地区：[1]湖南中医药大学第一附属医院放射科,湖南长沙410007 [2]怀化市中医医院影像科,湖南怀化418099

出　　处：《中国医学影像技术》2022年第10期1446-1451,共6页Chinese Journal of Medical Imaging Technology

基　　金：湖南中医药大学“十三五”一级学科(4901-020000200806);湖南省科技人才托举工程项目-年轻优秀科技工作者培养计划(2022TJ-N05)。

摘　　要：目的观察3.0T MR T1ρ及T2 mapping评估兔股骨内侧髁关节软骨退变的价值。方法将40只兔随机分为2周组、4周组、6周组及对照组各10只。对2周组、4周组及6周组兔建立右后肢膝骨关节炎模型并分别制动2、4及6周,对照组不予处理;行T1ρ及T2 mapping成像。处死动物后取股骨内侧髁关节软骨病变最严重区域行病理切片及染色;根据国际骨关节炎研究学会(OARSI)分级标准分为正常组、退变早期组及退变中晚期组,测定Ⅱ型胶原、蛋白多糖、β-Catenin及基质金属蛋白酶(MMP)-13,观察T1ρ及T2 mapping评估其关节软骨退变的价值。结果最终对37只兔造模成功,正常组8只、早期退变组20只、中晚期退变组9只;3组股骨内侧髁关节软骨T1ρ值及T2值差异均有统计学意义(P均<0.05),两两比较差异亦有统计学意义(P均<0.05)。T1ρ值及T2值与OARSI分级(r=0.72、0.73,P均<0.01)、MMP-13表达(r=0.84、0.59,P均<0.01)及β-Catenin表达(r=0.76、0.66,P均<0.01)均呈正相关,与Ⅱ型胶原含量(r=-0.70、-0.61,P均<0.01)及蛋白多糖含量(r=-0.82、-0.57,P均<0.01)均呈负相关。对正常组及退变早期组共28只兔行logistic回归分析,T1ρ值联合T2值鉴别兔正常与早期右后肢股骨内侧髁关节软骨退变的曲线下面积为0.84,敏感度及特异度分别为90.40%及75.26%。结论3.0T MR T1ρ及T2 mapping可定量评估兔股骨内侧髁关节软骨退变,用于判断其早期退变较具价值。Objective To observe the value of 3.0T MR T1ρand T2 mapping for evaluating articular cartilage degeneration of medial femoral condyle in rabbits.Methods Forty rabbits were randomly divided into 2-week group,4-week group,6-week group and control group(each n=10).The knee osteoarthritis models of the right hind limb were established in 2-week,4-week and 6-week group with braking time of 2,4 and 6 weeks,respectively.T1ρand T2 mapping of the right hind knee joint were performed.The rabbits were then sacrificed,the most serious areas of articular cartilage lesion of medial femoral condyle were taken for pathological section and staining.The samples were divided into normal group,early degeneration group,middle and late degeneration group according to Osteoarthritis Research Society International(OARSI),and typeⅡcollagen,proteoglycan,β-Catenin and matrix metalloproteinase(MMP)-13 were detected.The value of T1ρand T2 mapping for evaluating articular cartilage degeneration were analyzed.Results The models were successfully established in 37 rabbits,including 8 in normal group,20 in early degeneration group and 9 in middle and late degeneration group.T1ρand T2 values of articular cartilage of medial femoral condyle of the right hind limb were significant different among 3 groups(all P<0.05),as well as between each 2 groups(all P<0.05).T1ρand T2 values were positively correlated with OARSI grade(r=0.72,0.73,both P<0.01),MMP-13(r=0.84,0.59,both P<0.01)andβ-Catenin expression(r=0.76,0.66,both P<0.01),while negatively correlated with typeⅡcollagen(r=-0.70,-0.61,both P<0.01)and proteoglycan(r=-0.82,-0.57,both P<0.01).Logistic regression analysis was performed based on 28 rabbits of normal group and early degeneration group,and the results showed that the area under the curve of T1ρvalue combined with T2 value for differentiating articular cartilage of the medial femoral condyle of the right hind limb being normal or in early degeneration stage was 0.84,with sensitivity of 90.40%and specificity of 75.26%,respectivel

关 键 词：骨关节炎 膝 软骨 关节 磁共振成像 

分 类 号：R681.3[医药卫生—骨科学] R445.2[医药卫生—外科学]