黄连提取物对重症急性胰腺炎大鼠肠道菌群和黏膜屏障功能的影响研究  

Effect of coptidis extract on the intestinal microflora and mucosal barrier function of rats with severe acute pancreatitis

作　　者：艾飞艳 王文豪 邓敏子 刘锐[1] AI Fei-yan;WANG Wen-hao;DENG Min-zi;LIU Rui(Department of Gastroenterology,The Third Xiangya Hospital,Central South University,Changsha 410013,Hunan Province,China;Department of Breast and Nail Surgery,The First Affiliated Hospital of Nanhua University,Hengyang 421000,Hunan Province,China)

机构地区：[1]中南大学湘雅三医院消化科,湖南长沙410013 [2]南华大学附属第一医院乳甲外科,湖南衡阳421000

出　　处：《中国临床药理学杂志》2022年第18期2192-2196,共5页The Chinese Journal of Clinical Pharmacology

基　　金：湖南省自然科学基金资助项目(2019JJ50549)。

摘　　要：目的研究黄连提取物对重症急性胰腺炎(SAP)大鼠肠道菌群、黏膜屏障功能的影响,并探讨相关机制。方法将36只大鼠建立SAP模型,随机分为模型组、实验A组、实验B组,每组12只。建模结束后剔除建模失败大鼠,最终模型组、实验A组、实验B组均纳入10只。10只仅做假手术,设为对照组。实验A组给予黄连提取物300 mg·kg^(-1)灌胃,实验B给予黄连提取物300 mg·kg^(-1)+脂多糖(LPS)100 mg·kg^(-1)灌胃,对照组、模型组给予等体积生理盐水灌胃,每天1次,连续干预5天。用实时荧光定量逆转录聚合酶链反应(qRT-PCR)法检测肠内菌群;用放射免疫分析法检测血清内毒素(ET)水平以观察肠道黏膜屏障功能;用蛋白质印迹法检测胰腺组织Toll样受体4(TLR4)、髓样分化因子88(MyD88)、核转录因子κB p65(NF-κB p65)、磷酸化NF-κB p65(p-NF-κb p65)蛋白表达。结果对照组、模型组、实验A组、实验B组乳酸杆菌数量分别为(8.69±0.93),(5.77±0.68),(7.51±0.65),(6.60±0.72)lgCFU·g^(-1),大肠杆菌数量分别为(6.18±0.71),(8.74±0.92),(7.02±0.79),(7.85±0.95)lgCFU·g^(-1);血清ET分别为(30.25±4.18),(126.45±16.95),(70.62±9.23),(91.06±10.24)mg·mL^(-1);TLR4蛋白表达水平分别为0.10±0.02,0.46±0.05,0.23±0.03,0.35±0.04;MyD88蛋白表达水平分别为0.11±0.03,0.86±0.09,0.20±0.04,0.37±0.04;p-NF-κB p65/NF-κB p65分别为0.20±0.03,0.75±0.08,0.38±0.04,0.59±0.06。上述指标,对照组与模型组比较,实验A、B组与模型组比较,实验A组与实验B组比较,差异均有统计学意义(均P<0.05)。结论黄连提取物可改善SAP大鼠肠道菌群、黏膜屏障功能,减轻肠道及胰腺病理变化,可能通过抑制TLR4/MyD88信号通路来实现。Objective To explore the effect of coptis extract on the intestinal flora and mucosal barrier function of rats with severe acute pancreatitis(SAP),and to explore related mechanisms.Methods A total of 35 rats were established SPA model,and were randomly divided into model group(12 rats),experimental A group(12 rats)and experimental B group(11 rats).After modeling,the rats that failed to be modeled were eliminated,and finally 10 rats were included in model group,experimental A group,and experimental B group.Ten rats only underwent sham operation were set as control group.Experimental A group was administered with coptidis extract 300 mg·kg^(-1),by gavage and experimental-B group was administered with coptidis extract 300 mg·kg^(-1)+lipopolysaccharide(LPS)100 mg·kg^(-1)by gavage.Control group and model group were administered with equal volume of normal saline,once a day,continuous intervention for 5 days.Real-time fluorescent quantitative polymerase chain reaction(qRT-PCR)was used to detect intestinal flora.Serum endotoxin(ET)levels were measured by radioimmunoassay to observe intestinal mucosal barrier function.Western blotting was used to detect toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88),nuclear factorκB p65(NF-κB p65),phosphorylated NF-κB p65(p-NF-κB p65)protein expression in pancreatic tissue.Results The number of Lactobacilli in control group,model group,experimental A group and experimental B group were(8.69±0.93),(5.77±0.68),(7.51±0.65),(6.60±0.72)lg CFU·g^(-1);the number of Escherichia coli were(6.18±0.71),(8.74±0.92),(7.02±0.79),(7.85±0.95)lg CFU·g^(-1);the serum ET were(30.25±4.18),(126.45±16.95),(70.62±9.23),(91.06±10.24)mg·mL^(-1);the TLR4 protein expression levels were 0.10±0.02,0.46±0.05,0.23±0.03,0.35±0.04;the MyD88 protein expression levels were 0.11±0.03,0.86±0.09,0.20±0.04,0.37±0.04;the p-NF-κB p65/NF-κB p65 were 0.20±0.03,0.75±0.08,0.38±0.04,0.59±0.06.The above indexes compared between control group and model group,between experimental

关 键 词：重症急性胰腺炎 黄连提取物 肠道菌群 黏膜屏障功能 

分 类 号：R97[医药卫生—药品]