合成大麻素5F-EMB-PICA在斑马鱼体内潜在代谢标志物的筛选  

Metabolic markers of the synthetic cannabinoid 5F-EMB-PICA in zebrafish

作　　者：李佳瑞 王继芬[1] 张文芳[2] 黑海 LI Jiarui;WANG Jifen;ZHANG Wenfang;HEI Hai(College of Criminal Science and Technology,People's Public Security University of China,Beijing 100038,China;The Judicial Identification Center of Beijing Public Security,Key Laboratory of Forensic Toxicology,Ministry of Public Security,Beijing 100192,China)

机构地区：[1]中国人民公安大学侦查学院,北京100038 [2]北京市公安局司法鉴定中心法庭毒物分析公安部重点实验室,北京100192

出　　处：《分析试验室》2022年第9期1041-1046,共6页Chinese Journal of Analysis Laboratory

基　　金：中国人民公安大学基本科研业务费重点项目(2021JKF208)资助。

摘　　要：利用斑马鱼实验模型,借助液相色谱组合型四极杆Orbitrap质谱联用技术对N-(1-乙氧基羰基-2-甲基丙基)-1-(5-氟戊基)吲哚-3-甲酰胺(5F-EMB-PICA)的体内代谢产物进行分析,筛选潜在代谢标志物。结果表明,在斑马鱼实验模型中5F-EMB-PICA经过代谢共产生13种Ⅰ相代谢产物和6种Ⅱ相代谢产物。Ⅰ相代谢的途径包括酰胺水解、氟戊烷基侧链氧化脱氟、N-脱烷基化、羟基化和酯水解等。Ⅱ相代谢的途径主要为葡萄糖醛酸化。其中酯水解代谢产物(M5.1)响应强度最高,推荐作为5F-EMB-PICA的潜在代谢标志物。为建立生物样品中5F-EMB-PICA的标准检验方法提供相关代谢研究数据,为相关案件的检验鉴定提供技术支持。By using the zebrafish as experimental model,the in vivo metabolites of ethyl N-{[1-(5-fluoropentyl)-1H-indol-3-yl]carbonyl}-L-valinate(5F-EMB-PICA)were analyzed by using the Q ExactiveHF Quadrupole-Orbitrap LC-MS to screen potential metabolic markers.The results showed that a total of 13 phaseⅠmetabolites and 6 phaseⅡmetabolites were generated.The pathways of phaseⅠmetabolism included amide hydrolysis,fluoropentyl oxidative defluorination,N-dealkylation,hydroxylation and ester hydrolysis.The main pathway of phaseⅡmetabolism was glucuronidation.Among them,the ester hydrolysis metabolite(M5.1)provided the highest response intensity,thus it was recommended as a potential metabolic marker of 5F-EMB-PICA.It aims to provide relevant metabolic research data for the establishment of a standard test method for 5F-EMB-PICA in biological samples,and technical support for the test and identification of related cases.

关 键 词：5F-EMB-PICA 合成大麻素 斑马鱼 代谢途径 

分 类 号：R917[医药卫生—药物分析学]