基于抗氧化作用观察胃血止糊剂对应激性溃疡模型大鼠的保护作用和机制  

Study on protection and mechanism of gastric blood check paste on stress ulcer in model rats:based on antioxidation

作　　者：许云姣 吴文笛[1] 张志毕[2] 蔡悦青 张兰春[2] 周瑞彬[1] 杨翼豪 姜莉云[1] XU Yunjiao;WU Wendi;ZHANG Zhibi;CAI Yueqing;ZHANG Lanchun;ZHOU Ruibin;YANG Yihao;JIANG Liyun(Department of Spleen,Stomach and Liver Diseases,Kunming Municipal Hospital of Traditional Chinese Medicine,Kunming,Yunnan 650011;Institute of Biomedical Engineering,Kunming Medical University,Kunming,Yunnan 650500)

机构地区：[1]云南省昆明市中医医院脾胃肝病科,云南昆明650011 [2]昆明医科大学生物医学工程研究院,云南昆明650500

出　　处：《河北中医》2022年第8期1335-1341,共7页Hebei Journal of Traditional Chinese Medicine

基　　金：云南省科学技术厅-中医联合专项青年项目[编号:2018FF001(-084)];云南省科学技术厅-中医联合专项重点项目(编号:202101AZ070001-007);云南省科学技术厅-中医联合专项面上项目(编号:202101AZ070001-088);昆明市卫生科技人才培养项目暨许云姣“千”工程后备人选项目[编号:2020-SW(后备)-87];昆明市卫生健康委员会卫生科研局级项目(编号:2020-03-04-001)。

摘　　要：目的观察胃血止糊剂对大鼠应激性溃疡(SU)的保护作用和机制。方法将72只SPF级SD雄性大鼠随机分为空白组、模型组、奥美拉唑组及胃血止糊剂高、中、低剂量组共6组,每组12只,奥美拉唑组予奥美拉唑5 mg/(kg·d)灌胃,胃血止糊剂高、中、低剂量组分别予胃血止糊剂4、2、1 g/(kg·d)灌胃,空白组和模型组予等容积的溶媒灌胃,每日1次,共灌胃14 d。灌胃后除空白组外,其余组采用“水浸束缚应激”制备SU模型。采用激光多普勒测定各组大鼠建模后胃黏膜血流量,制作苏木素-伊红染色切片观察各组胃黏膜组织病理形态学变化;采用酶联免疫吸附法检测各组大鼠血清及胃黏膜组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)活性及丙二醛(MDA)含量。结果与空白组比较,模型组、奥美拉唑组和胃血止糊剂高、中、低剂量组GMBF均降低(P<0.05),UI均升高(P<0.05);与模型组比较,奥美拉唑组和胃血止糊剂高、中、低剂量组GMBF均升高(P<0.05),奥美拉唑组和胃血止糊剂高、中剂量组UI均降低(P<0.05);与奥美拉唑组、胃血止糊剂高剂量组比较,胃血止糊剂中、低剂量组GMBF均降低(P<0.05),与奥美拉唑组比较,胃血止糊剂中、低剂量组UI均升高(P<0.05),胃血止糊剂高剂量组GMBF、UI与奥美拉唑组比较差异无统计学意义(P>0.05);与胃血止糊剂中剂量组比较,胃血止糊剂低剂量组GMBF显著降低(P<0.05),UI无差异(P>0.05)。空白组大鼠胃黏膜组织未见损伤性的变化,胃黏膜结构完整,腺体排列整齐;模型组大鼠胃黏膜损伤明显,上皮细胞广泛坏死脱落,腺体结构遭到破坏,形成多个大小、深浅不等的糜烂面。奥美拉唑组和胃血止糊剂各剂量组胃黏膜损伤较模型组明显减轻。与空白组比较,模型组、奥美拉唑组及胃血止糊剂高、中、低剂量组大鼠血清SOD、GSH-Px、CAT活性均降低(P<0.05),MDA含量均升高(PObjective To observe the protection and mechanism of gastric blood check paste on stress ulcer(SU)in rats.Methods Totally 72 SPF-grade SD male rats randomly assigned into the blank group,the model group,the omeprazole group,the high-dose,medium-dose and low-dose gastric blood check paste groups,with 12 per group.The omeprazole group was given omeprazole(5 mg/[kgod])by gavage;the high-dose,medium-dose and low-dose gastric blood check paste groups were given gastric blood check paste(4,2,and 1 g/[kgod],respectively)by gavage;the blank group and the model group received equal volume of dissolvent;the treatment continued for 14 days(qd).The SU model was established by water-immersion stress in the other groups apart from the blank group.The gastric mucosal blood flow was measured with laser Doppler flowmeter;the pathomorphism of gastric mucosa was observed using hematoxylin-eosin-stained sections;the superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),Catalase(CAT),malonaldehyde(MDA)of serum and gastric mucosa were determined by enzyme-linked immunosorbent assay(ELISA).Results In comparison of the blank group and the other groups,the other groups displayed decreased gastric mucosal blood flow(GMBF),and increased ulcer index(UI)(all P<0.05);compared with the model group,GMBF in the omeprazole group,the high-,medium-and low-dose gastric blood check paste groups was increased(P<0.05),UI in the omeprazole group,the high-,medium-dose gastric blood check paste groups was decreased(P<0.05);lower GMBF were detected in the medium-and low-dose gastric blood check paste groups than those in the the omeprazole group,the high-dose gastric blood check paste group,and higher UI were detected in the medium-and low-dose gastric blood check paste groups than those in the the omeprazole group(all P<0.05);none of the GMBF and UI differed significantly between the high-dose gastric blood check paste group and the omeprazole group(P>0.05);GMBF in the low-dose gastric blood check paste group was significantly decreased than that in th

关 键 词：消化性溃疡 大鼠 中药疗法 超氧化物歧化酶 过氧化氢酶 动物实验 

分 类 号：R285.5[医药卫生—中药学] R573.130.531[医药卫生—中医学]