MiR-140 downregulates fatty acid synthesis by targeting transforming growth factor alpha(TGFA)in bovine mammary epithelial cells  

作　　者：CHU Shuang-feng ZHAO Tian-qi Abdelaziz Adam Idriss ARBAB YANG Yi CHEN Zhi YANG Zhang-ping 

机构地区：[1]College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,P.R.China [2]Joint International Research Laboratory of Agriculture&Agri-Product Safety,Yangzhou University,Yangzhou 225009,P.R.China [3]Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,P.R.China

出　　处：《Journal of Integrative Agriculture》2022年第10期3004-3016,共13页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31802035, 31872324 and 31601915)

摘　　要：Fat is an indispensable nutrient and basic metabolite for sustaining life,and milk is particularly rich in fatty acids,including a variety of saturated and unsaturated fatty acids.MicroRNA(miRNA)and mRNA play an important role in the regulation of milk fat metabolism in mammary gland tissue.It has been shown that lipid metabolism has a complex transcriptional regulation,but the mechanism by which milk fat synthesis is regulated through miRNA–mRNA interactions is poorly understood.In this study,we performed transcriptome sequencing with bovine mammary gland tissue in the late lactation(270 and 315 days after parturition)to identify the key gene that regulating milk fat metabolism.A total of 1207 differentially coexpressed genes were selected,828 upregulated genes and 379 downregulated genes were identified.The transforming growth factor alpha(TGFA)gene was selected as the target gene,and luciferase reporter assay,Western blotting and q RT-PCR were used for further study.The results demonstrated that miR-140 was an upstream regulator of TGFA,and miR-140 could inhibit(P<0.01)unsaturated fatty acid and triglyceride(TAGs)production in bovine mammary epithelial cells(BMECs).In contrast,TGFA promoted(P<0.01)unsaturated fatty acid and TAG production.Rescue experiments further indicated the mi R-140/TGFA regulatory mechanism.Taken together,these results suggest that the mi R-140/TGFA pathway can inhibit(P<0.01)milk fat metabolism and improve milk quality by genetic means.

关 键 词：bovine mammary epithelial cells TGFA transcriptome sequencing miR-140 fatty acid metabolism 

分 类 号：S823.2[农业科学—畜牧学]