丹参水溶性提取物丹参酚酸B的制备及应用  

Study on the preparation and application of salvianolic acid B,a water-soluble extract of Salvia miltiorrhiza

作　　者：陈萍萍 许全升 赵丽娜[1] 张晓霞[1] Pingping Chen;Quansheng Xu;Lina Zhao;Xiaoxia Zhang(Henan Provincial Engineering Research Center of Natural Drug Extraction and Medical Technology Application,Zhengzhou Railway Vocational&Technical College,Zhengzhou,Henan 450000,China;HeNan RunHong Pharmaceutical Co.,Ltd,Zhengzhou,Henan 450000,China)

机构地区：[1]郑州铁路职业技术学院河南省天然药物提取和医疗技术应用工程研究中心,河南郑州450000 [2]河南润弘制药股份有限公司,河南郑州450000

出　　处：《日用化学工业（中英文）》2022年第10期1100-1106,共7页China Surfactant Detergent & Cosmetics

基　　金：2022年度河南省高等学校重点科研项目(22A360018);2019年河南省医学教育研究项目(Wjlx2019260)。

摘　　要：探讨丹参水溶性提取物丹参酚酸B的制备及其生物转化应用研究。以丹参酚酸B的出膏率和提取率为评价指标,比较丹参水溶性提取法包括水煎煮回流法、超声提取法、渗漉提取法,采用高效液相色谱(HPLC)法测定。研究发现,常规水煎煮回流法中丹参酚酸B提取率最高可达72.59%,远高于超声提取法的63.72%和渗漉提取法的65.69%(P<0.05)。丹参酚酸B的生物转化应用结果显示,100 g丹参酚酸B可以制备出14.2 g丹参素(纯度≥95%)和35.8 g Prewalskinic acid A(纯度≥91%),转化效率高。对丹参水溶性提取物丹参酚酸B的制备工艺进行了相关研究,获得了简便、高效、科学的工艺路线,该研究为丹参的进一步开发和实际应用奠定了理论和实践基础。The purpose was to study the preparation and biotransformation application of salvianolic acid B,a water-soluble extract of Salvia miltiorrhiza.The extraction rate and extraction rate of salvianolic acid B were used as evaluation indicators to compare the water-soluble extraction methods of Salvia miltiorrhiza,including water decoction reflux method,ultrasonic extraction method,percolation method,etc.,which were determined by high performance liquid chromatography(HPLC).After repeated exploration and comparison,the study finds that the extraction rate of salvianolic acid B in the conventional water decoction reflux method is as high as 72.59%,which is much higher than of the ultrasonic extraction method of 63.72%and the percolation method of 65.69%(P<0.05).In the application of biotransformation of salvianolic acid B,the study finds that 100 g of salvianolic acid B can yield 14.2 g of Danshensu(purity≥95%)and 35.8 g of Prewalskinic acid A(purity≥91%),which indicates that the biotransformation is very successful.In this study,we have conducted related research on the preparation process of salvianolic acid B,a water-soluble extract of Salvia miltiorrhiza,obtained a simple,efficient and scientific process route,and studied its biotransformation application,which found a high conversion efficiency of salvianolic acid B into Przewalskinic acid A and Danshensu.This research lays a theoretical and practical foundation for the further development and practical application of Salvia miltiorrhiza.

关 键 词：丹参 水煎煮回流法 丹参酚酸B 丹参素 

分 类 号：TQ658[化学工程—精细化工]