宫腔粘连患者血清miR-214、CTGF表达及预测术后复发价值  

Expression levels of serum miR-214 and connective tissue growth factor of women with intrauterine adhesions and their value for predicting the postoperative adhesions recurrence

作　　者：马飞雯 沙红兰 马宗丽 李寅红 仇菊[1] MA Feiwen;SHA Honglan;MA Zongli;LI Yinhong;QIU Ju(Rugao People's Hospital,Jiangsu Province,226500)

机构地区：[1]江苏省如皋市人民医院,226500

出　　处：《中国计划生育学杂志》2022年第10期2238-2242,2247,共6页Chinese Journal of Family Planning

摘　　要：目的:探讨血清miR-214和结缔组织生长因子(CTGF)表达情况与宫腔粘连(IUA)临床病理因素以及术后复发的关系.方法:收集本院2019年9月-2020年9月收治的IUA患者80例为IUA组,均行宫腔粘连分离术;健康体检者60例为对照组.应用qRT-PCR检测两组血清miR-214表达,酶联免疫吸附法检测血清CTGF水平;pearson法分析血清miR-214与CTGF水平的相关性;分析IUA患者术前与术后血清miR-214、CTGF表达变化;受试者工作特征(ROC)曲线分析miR-214、CTGF表达水平对IUA术后复(12例)发的预测价值;多因素logistic回归分析IUA患者术后复发的危险因素.结果:IUA组血清miR-214水平(0.46±0.08)低于对照组(1.24±0.16),CTGF水平(141.35±34.18ng/ml)高于对照组(66.75±17.48ng/ml)(均P<0.05),血清miR-214与CTGF表达呈负相关(r=-0.538,P<0.05).IUA组患者术前与术后血清miR-214、CTGF表达水平差异显著(P<0.05).ROC曲线分析显示,miR-214、CTGF预测IUA患者术后复发的曲线下面积分别为0.787、0.823,敏感性分别为75.0%、66.7%,特异性分别为91.2%、91.2%.多因素分析显示,人工流产>3次、宫腔粘连性质为肌性、宫腔粘连范围>2/3、miR-214低表达、CTGF高表达是IUA患者术后复发的独立危险因素(P<0.05).结论:IUA患者血清miR-214、CTGF表达异常,且对术后复发有一定诊断价值,有望成为预测IUA患者不良预后的生物学指标.Objective: To investigate the correlation between the levels of serum miR-214 and connective tissue growth factor(CTGF) of women with intrauterine adhesions(IUA) and their clinicopathological factors and postoperative IUA recurrence. Methods: A total of 80 women with IUA were collected in study group and 60 healthy women without IUA who wanted physical examination were selected in control group from September 2019 to September 2020. qRT-PCR was used to detect the level of serum miR-214, and enzyme-linked immunosorbent(ELISA) method was used to detect the expression level of serum CTGF. Pearson method was used to analyze the correlation between the serum miR-214 level of the women and their CTGF level. The different levels of serum miR-214 and CTGF expressions of the women before and after surgery were analyzed. Receiver operating characteristic(ROC) curve was used to analyze the value of miR-214 and CTGF expression levels for predicting the postoperative recurrence of IUA. Multivariate Logistic regression was used to analyze the risk factors of the postoperative recurrence of IUA. Results: The level of serum miR-214(0.46±0.08) of the women in the study group was significantly lower than that(1.24±0.16) of the women in control group, and the level of CTGF(141.35±34.18 ng/ml) of the women in the study group was significantly higher than that(66.75±17.48 ng/ml) of the women in the control group(all P<0.05). The serum miR-214 level of the women was negatively correlated with their CTGF level(r=-0.538,P<0.05).The levels of serum miR-214and CTGF of the women in the study group before surgery and had significantly different from those after surgery(P<0.05).ROC curve analysis showed that the area under the curve,the sensitivity,and the specificity of the serum miR-214level for predicting postoperative IUA recurrence of the patients were 0.787,66.7%,and 91.2%,respectively,and which of the serum CTGF level for predicting postoperative IUA recurrence of the patients were 0.823,75.0%,and91.2%,respectively.Multivariate an

关 键 词：宫腔粘连 宫腔粘连分离术 miR-214 结缔组织生长因子 术后复发 预测 

分 类 号：R713.4[医药卫生—妇产科学]