LncRNA MALAT1和miR141在子痫前期孕妇胎盘及血清中表达及临床意义  

Expression levels and clinical significance of long non-coding RNA lung adenocarcinoma metastasis-associated transcript-1 and microRNA-141 in placenta and serum of pregnant women with preeclampsia

作　　者：陈芳荣[1] 陈小菊[1] CHEN Fangrong;CHEN Xiaoju(Hainan Provincial People's Hospital(Hainan Hospital Affiliated to Hainan Medical College),Haikou,Hainan Province,570311)

机构地区：[1]海南省人民医院(海南医学院附属海南医院),海口570311

出　　处：《中国计划生育学杂志》2022年第10期2353-2357,共5页Chinese Journal of Family Planning

基　　金：海南省自然科学基金面上项目(821MS128);海南省自然科学基金面上项目(822MS164);海南省人民医院院级国家自然科学基金培育530工程面上项目(2021MSXM04)。

摘　　要：目的:探讨长链非编码RNA(LncRNA)肺腺癌转移相关转录产物-1(MALAT1)和微小RNA-141(miR141)在子痫前期(PE)孕妇胎盘及血清的表达及临床意义.方法:选取2019年5月-2020年5月本院住院治疗的PE孕妇45例为PE组,根据孕妇病情分为PE组(n=24)和重度PE组(n=21),同期产前检查正常孕妇45例为对照组,检测两组孕妇胎盘及血清中LncRNA MALAT1、miR141表达水平并分析相关性,受术者工作特征曲线(ROC)分析2项指标对不良妊娠结局的诊断价值.结果:重度PE组胎盘和血清中LncRNA MALAT1(0.58±0.19、0.37±0.16)低于PE组(0.76±0.20、0.52±0.17)和对照组(1.03±0.24、0.94±0.23),miR141(1.52±0.27、1.40±0.28)高于PE组(1.19±0.25、1.26±0.24)和对照组(0.73±0.18、1.06±0.20),且PE组胎盘和血清LncRNA MALAT1与miR141表达均呈负相关;PE组不良妊娠结局孕妇胎盘和血清LncRNA MALAT1均低于妊娠结局良好组,miR141表达高于妊娠结局良好组(均P<0.05).ROC曲线显示,胎盘LncRNA MALAT1、miR141联合预测PE孕妇不良妊娠结局的曲线下面积(AUC=0.913)高于LncRNA MALAT1(0.804)、miR141(0.750)单独检测(P<0.05),血清LncRNA MALAT_(1)、miR141联合预测不良妊娠结局的AUC(0.929)高于LncRNA MALAT1(0.732)、miR141(0.805)单独检测(均P<0.05).结论:PE孕妇胎盘及血清中的LncRNA MALAT1表达下降、miR141表达升高,且与病情进展相关,2项指标联合检测可用于PE孕妇不良妊娠结局的预测诊断.Objective: To explore the expression levels and clinical significance of long non-coding RNA(LncRNA) lung adenocarcinoma metastasis-associated transcript-1(MALAT1) and microRNA-141(miR 141) in placenta and serum of pregnant women with preeclampsia(PE). Methods: A total of 45 pregnant women with PE were enrolled in study group and were divided into group A(24 women with mild PE) and group B(21 women with severe PE) according to the severity of their PE between May 2019 and May 2020. Another 45 normal pregnant women undergoing physical examination were enrolled in control group during the same period. The expression levels of LncRNA MALAT1 and miR 141 in placenta and serum of these women were detected, and the correlation between the LncRNA MALAT1 level and the miR 141 level was statistically analyzed. The diagnostic value of the LncRNA MALAT1 level combined with the miR 141 level for adverse pregnancy outcomes of the women with PE was analyzed by receiver operator characteristic(ROC) curve. Results: The levels of LncRNA MALAT1 in placenta and serum(0.58±0.19, 0.37±0.16) of the women in group B were significantly lower than those(0.76±0.20,0.52±0.17)of the women in group A and those(1.03±0.24,0.94±0.23)of the women in the control group.The levels of miR 141in placenta and serum(1.52±0.27,1.40±0.28)of the women in group B were significantly higher than those(1.19±0.25,1.26±0.24)of the women in group A,and those(0.73±0.18,1.06±0.20)of the women in the control group.The expression levels of LncRNA MALAT1in placenta and serum of the women in group A were negatively correlated with their miR 141levels in placenta and serum.In group A,the levels of LncRNA MALAT1in placenta and serum of the women with adverse pregnancy outcomes were significantly lower than those women with normal pregnancy outcomes,and the miR141levels in placenta and serum of the women with adverse pregnancy outcomes were significantly higher(all P<0.05).ROC curve showed that the area under the curve(AUC)of the LncRNA MALAT1level combined wi

关 键 词：子痫前期 肺腺癌转移相关转录产物-1 微小RNA-141 不良妊娠 预测 

分 类 号：R714.244[医药卫生—妇产科学]