某种鸡群沙门菌的分离鉴定、毒力基因检测及单宁酸抑菌作用分析  

Isolation,identification,virulence gene detection and antimicrobial activity of tannic acid of Salmonella from chicken

作　　者：刘盼盼 王磊[1] 王玲[1] 贺泂杰[1] 孙研 张长安 王胜义[1] 崔东安[1] LIU Pan-pan;WANG Lei;WANG Ling;HE Jiong-jie;SUN Yan;ZHANG Chang-an;WANG Sheng-yi;CUI Dong-an(Lanzhou Institute of Husbandry and Pharmaceutical Sciences,Chinese Academy of Agricultural Sciences,Engineering Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province,Lanzhou 730050,China;College of Agriculture,Ningxia University,Yinchuan 750021,China;Wufeng Chicheng Biotech Limited Liability Company,Yichang 443413,China)

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所,甘肃省中兽药工程技术研究中心,甘肃兰州730050 [2]宁夏大学,宁夏银川750021 [3]五峰赤诚生物科技股份有限公司,湖北宜昌443413

出　　处：《中国兽医科学》2022年第9期1191-1198,共8页Chinese Veterinary Science

基　　金：国家自然科学基金项目(31902316);甘肃省青年科技基金项目(20JR5RA575);甘肃省重点研发项目(20YF3NA003);中国农业科学院基本科研业务费专项(1610322020012)。

摘　　要：为了解宁夏某种鸡群孵化率及雏鸡存活率下降原因,随机采集血样进行鸡白痢、鸡伤寒抗体血清学检测,同时采集死胚及死雏肝进行病原菌分离。分离病原菌通过16S rRNA和生化试验,经多重PCR鉴定禽源沙门菌血清型并检测了14种毒力基因,测定分离株对单宁酸(TA)的最低抑菌浓度(MIC)。结果显示,鸡群鸡白痢、鸡伤寒抗体阳性率为20%;试验共分离病原菌39株,其中鸡源沙门菌29株,主要血清型为鸡白痢沙门菌26株、肠炎沙门菌2株、鼠伤寒沙门菌1株;鸡白痢沙门菌对单宁酸最为敏感,MIC为0.125~1 mg/m L。本研究揭示了该种鸡群内病原菌感染情况和携带的沙门菌毒力基因,检测了单宁酸对各分离株的MIC,为单宁酸净化沙门菌的临床应用提供了参考。To understand the reasons for the decrease in hatching rate and chick survival in a certain flock,blood samples were randomly collected for serological testing of antibodies to chicken dysentery and chicken typhoid,and dead embryos and livers of dead chicks were collected for isolation of pathogenic bacteria.The isolated pathogens were preliminarily identified by 16S rRNA and biochemical tests.Multiplex PCR was used to identify Avian Salmonella serotypes and 14 virulence genes were detected.Furthermore,and the minimum inhibitory concentration(MIC)of the isolates against tannic acid was determined.The results showed that the antibody positive rate of chicken white dysentery and chicken typhoid was 20%.39strains of pathogenic bacteria were isolated,including 29 strains of Salmonella.The main serotypes were 26 strains of Salmonella pullorum,2 strains of Salmonella enteritidis and 1 strain of Salmonella typhimurium.Salmonella pullorum was the most sensitive to tannic acid with MIC of 0.125—1.00 mg/m L.This study revealed the pathogenic bacteria infection and virulence gene carrying of Salmonella in chicken flocks.We detected the MIC of tannic acid to each isolate.It provides reference for clinical application of tannic acid in Salmonella purification.

关 键 词：种鸡群 沙门菌 毒力基因 单宁酸 

分 类 号：S852.612[农业科学—基础兽医学]