Integrated Au-Nanoroded Biosensing and Regulating Platform for Photothermal Therapy of Bradyarrhythmia  

作　　者：Jiaru Fang Dong Liu Dongxin Xu Qianni Wu Hongbo Li Ying Li Ning Hu 

机构地区：[1]State Key Laboratory of Optoelectronic Materials and Technologies,Guangdong Province Key Laboratory of Display Material and Technology,School of Electronics and Information Technology,State Key Laboratory of Ophthalmology,Zhongshan Ophthalmic Center,Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science,Sun Yatsen University,Guangzhou 510006,China [2]Stoddart Institute of Molecular Science,Department of Chemistry,ZJU-Hangzhou Global Scientific and Technological Innovation Center,Zhejiang University,Hangzhou 310058,China [3]Molecular Cancer Research Center,School of Medicine,Sun Yat-sen University,Shenzhen 518107,China [4]State Key Laboratory of Transducer Technology,Chinese Academy of Sciences,Shanghai 200050,China

出　　处：《Research》2022年第3期31-44,共14页研究（英文）

基　　金：the National Natural Science Foundation of China(Grant Nos.82061148011,62171483,and 621042640);Guangdong Basic and Applied Basic Research Foundation(Grant Nos.2020A1515010665,2020A1515110424,2020A1515111210,and 2021A1515011609);Department of Science and Technology of Guangdong Province Project(Grant No.2020B1212060030);Science and Technology Program of Guangzhou(Grant No.202102010231);Funds of the Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science(Grant No.2020B1212060033);Open Project of Chinese Academy of Sciences(Grant No.SKT2006).

摘　　要：Bradyarrhythmia is a kind of cardiovascular disease caused by dysregulation of cardiomyocytes,which seriously threatens human life.Currently,treatment strategies of bradyarrhythmia mainly include drug therapy,surgery,or implantable cardioverter defibrillators,but these strategies are limited by drug side effect,surgical trauma,and instability of implanted devices.Here,we developed an integrated Au-nanoroded biosensing and regulating platform to investigate the photothermal therapy of cardiac bradyarrhythmia in vitro.Au-nanoroded electrode array can simultaneously accumulate energy from the photothermal regulation and monitor the electrophsiological state to restore normal rhythm of cardiomyocytes in real time.To treat the cardiomyocytes cultured on Au-nanoroded device by near-infrared(NIR)laser irradiation,cardiomyocytes return to normal for long term after irradiation of suitable NIR energy and maintenance.Compared with the conventional strategies,the photothermal strategy is more effective and convenient to regulate the cardiomyocytes.Furthermore,mRNA sequencing shows that the differential expression genes in cardiomyocytes are significantly increased after photothermal strategy,which are involved in the regulation of the heart rate,cardiac conduction,and ion transport.This work establishes a promising integrated biosensing and regulating platform for photothermal therapy of bradyarrhythmia in vitro and provides reliable evidence of photothermal regulation on cardiomyocytes for cardiological clinical studies.

关 键 词：ARRHYTHMIA RETURN conduction 

分 类 号：R54[医药卫生—心血管疾病] TB383[医药卫生—内科学]