Solar-Driven Producing of Value-Added Chemicals with Organic Semiconductor-Bacteria Biohybrid System  

作　　者：Wen Yu Haotian Bai Yue Zeng Hao Zhao Shengpeng Xia Yiming Huang Fengting Lv Shu Wang 

机构地区：[1]Beijing National Laboratory for Molecular Sciences,Key Laboratory of Organic Solids,Institute of Chemistry,Chinese Academy of Sciences,Beijing 100190,China [2]College of Chemistry,University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Research》2022年第3期283-295,共13页研究（英文）

基　　金：the National Natural Science Foundation of China(Nos.22021002,22020102005,22022705,21773268);the National Key Research and Development Program of China(No.2018YFE0200700).

摘　　要：Photosynthetic biohybrid systems exhibit promising performance in biosynthesis;however,these systems can only produce a single metabolite and cannot further transform carbon sources into highly valuable chemical production.Herein,a photosynthetic biohybrid system integrating biological and chemical cascade synthesis was developed for solar-driven conversion of glucose to value-added chemicals.A new ternary cooperative biohybrid system,namely bacterial factory,was constructed by self-assembling of enzyme-modified light-harvesting donor-acceptor conjugated polymer nanoparticles(D-A CPNs)and genetically engineered Escherichia coli(E.coli).The D-A CPNs coating on E.coli could effectively generate electrons under light irradiation,which were transferred into E.coli to promote the 37%increment of threonine production by increasing the ratio of nicotinamide adenine dinucleotide phosphate(NADPH).Subsequently,the metabolized threonine was catalyzed by threonine deaminase covalently linking with D-A CPNs to obtain 2-oxobutyrate,which is an important precursor of drugs and chemicals.The 2-oxobutyrate yield under light irradiation is increased by 58%in comparison to that in dark.This work provides a new organic semiconductor-microorganism photosynthetic biohybrid system for biological and chemical cascade synthesis of highly valuable chemicals by taking advantage of renewable carbon sources and solar energy.

关 键 词：INCREMENT LINKING DONOR 

分 类 号：Q50[生物学—生物化学]