野生薄皮木组培快繁体系优化  

Optimization of Tissue Culture and Rapid PropagationSystem in Leptodermis oblonga Bunge

作　　者：郭丽[1] 朱飞雪[1] 寇艳玲[1] 常介田[1] GUO Li;ZHU Feixue;KOU Yanling;CHANG Jietian(He’nan Vocational College of Agriculture,Zhengzhou 451450,China)

机构地区：[1]河南农业职业学院,郑州451450

出　　处：《种子》2022年第9期139-145,共7页Seed

基　　金：河南省科技攻关重点研发与推广专项(212102110188);河南农业职业学院科研创新人才项目(HNACSRHR-2021-04);河南省高等教育教学改革研究与实践项目(2021SJGLX650)

摘　　要：为开发野生薄皮木在园林绿化中的应用,以野生薄皮木带腋芽茎段为试材,采用组织培养法,研究不同消毒剂种类和消毒时间对外植体萌芽的影响及不同植物生长调节剂种类及浓度对腋芽增殖、生根培养的影响,以期建立野生薄皮木组培快繁体系。结果表明,野生薄皮木带腋芽茎段适宜用75%酒精消毒20 s、5%NaClO消毒12 min,最佳增殖培养基为MS+ZT 1.0 mg/L,最佳生根培养基为1/2 MS+IBA 0.1 mg/L+NAA 0.05 mg/L,生根苗在草炭∶蛭石∶珍珠岩=3∶1∶1基质中,成活率达90%以上。For the development of wild Leptodermis oblonga Bunge application in garden greening,with wild Leptodermis oblonga Bunge axillary bud stem section as the test materials,the tissue culture method was adopted,the different types of disinfectant and sterilization time foreign implant bud and the influence of different types of plant growth regulator and concentration on the influence of lateral bud multiplication and rooting culture,in order to establish a wild Leptodermis oblonga Bunge seed rapid propagation system.The results showed that the stem segments of wild Leptodermis oblonga Bunge axillary buds were suitable for disinfection with 75%alcohol for 20 s and 5%NaClO for 12 min.The optimal proliferation medium was MS+ZT 1.0 mg/L,and the optimal rooting medium was 1/2 MS+IBA 0.1 mg/L+NAA 0.05 mg/L.The survival rate of rooting seedlings in peat∶vermiculite∶perlite equalling 3∶1∶1 substrate was more than 90%.

关 键 词：野生薄皮木 茎段 组织培养 生长调节剂 

分 类 号：S685.99[农业科学—观赏园艺]