基于UPLC-Q-TOF-MS技术快速分析鉴定四川阿坝野生滇黄精的化学成分  

Rapid analysis and identification of the chemical constituents of wild Polygonatum kingianum Coll.et Hemsl in Sichuan Aba by UPLC-Q-TOF-MS^(E)

作　　者：姬凯悦 王宏鹏 杨健[2] 秦建春[3] 杨胜祥[4] 吕常江 黄俊 JI Kai-yue;WANG Hong-peng;YANG Jian;QIN Jian-chun;YANG Shen-xiang;LV Chang-jiang;HUANG Jun(Zhejiang University of Science and Technology,Zhejiang Provincial Key Laboratory for Chemistry and Biology Processing Technology of Farm Products,Zhejiang Province Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing,Hangzhou 310023,China;State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;College of Plant Sciences,Jilin University,Jilin Changchun 130062,China;Zhejiang Provincial Key Laboratory of Chemical Utilization of Forestry Biomass,Zhejiang A&F University,Linan 311300,China,China)

机构地区：[1]浙江科技学院生物与化学工程学院,浙江省农产品化学与生物加工技术重点实验室,浙江省农业生物资源生化制造协同创新中心,浙江杭州310023 [2]中国中医科学院中药资源中心,道地药材国家重点实验室培育基地,北京100700 [3]吉林大学植物科学学院,长春130062 [4]浙江农林大学浙江省林业生物质化学利用重点实验室,浙江临安311300

出　　处：《应用化工》2019年第S01期271-275,共5页Applied Chemical Industry

基　　金：浙江省科技厅分析测试项目(2018C37052);第48批教育部留学回国人员科研启动基金项目。

摘　　要：利用灵敏的超高效液相色谱-四极杆飞行时间质谱串联技术(UPLC-Q-TOF-MS)对珍稀中药资源四川阿坝野生滇黄精的80%甲醇提取液进行分析。采用电喷雾离子源(ESI)的阴离子模式对样品中的化学成分进行扫描,通过MS^(E)数据采集方式获得准分子离子峰及二级碎片等质谱信息。结合文献中已报道的黄精化学成分,建立了基于UNIFI平台的黄精数据库,通过快速筛选鉴定了包括5个黄酮类,17个甾体皂苷类等的28个化学成分,所有28个化合物为首次在四川阿坝野生滇黄精中确认,其中10个化合物为首次明确在滇黄精中发现。同时,对发现的黄酮类、皂苷类等的质谱裂解规律进行了探讨。该方法可用来快速、高效、准确评估黄精等中药活性成分。The chemical constituents from methanol extract of wild Polygonatum kingianum Coll.et Hemsl in Sichuan Aba were analyzed by ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) technology.The pseudo-molecular ions of the chemical constituents and their fragments were detected by the negative mode of electrospray ionization(ESI) and further MS^(E) data with low and high energy level.Under the guidance of the UNIFI database and the reported mass data,twenty-eight compounds were identified,including 5 flavonoids and 17 steroidal saponins.All 28 compounds were first identified in the wild Polygonatum kingianum Coll.et Hemsl in Sichuan Aba,and 10 of them were first identified in Polygonatum kingianum Coll.et Hemsl.This method can be used to quickly assess the differences and levels of compounds in wild Polygonatum kingianum Coll.et Hemsl in Sichuan Aba and further traditional Chinese medicines,and providing an accurate scientific basis for the determination and further development of Chinese herbal medicines.

关 键 词：滇黄精 高效液相色谱仪联用四极杆串联飞行时间质谱 UNIFI 化学成分 

分 类 号：TQ460.4[化学工程—制药化工] O629[理学—有机化学]