椒目油改善铁过载雌性去势大鼠骨质疏松的作用研究  

Zanthoxylum bungeanum Maxim seed oil inhibits osteoporosis in female iron overload and ovariectomized rats

作　　者：万思齐 王文志 左浩江[1] 邓羽丰 何方婷 罗姝菡 张保超 郑田利 许欣 裴晓方 WAN Si-qi;WANG Wen-zhi;ZUO Hao-jiang;DENG Yu-feng;HE Fang-ting;LUO Shu-han;ZHANG Bao-chao;ZHENG Tian-li;XU Xin;PEI Xiao-fang(West China School of Public Health and West China Fourth Hospital,Sichuan University,Chengdu,Sichuan 610041,China;不详)

机构地区：[1]四川大学华西公共卫生学院/华西第四医院,四川成都610041 [2]四川大学华西-协和陈志潜卫生健康研究院非传染性疾病(慢病)病因及防治研究中心

出　　处：《现代预防医学》2022年第19期3579-3584,共6页Modern Preventive Medicine

基　　金：四川省科技厅应用基础研究(2020YJ0233)。

摘　　要：目的椒目油(Zanthoxylum bungeanum Maxim seed oil,ZBSO)对铁过载雌性去势SD大鼠骨质疏松的保护作用及机制初探。方法将40只雌性SD大鼠随机分为假手术组(SHAM)、去势组(OVX)、铁过载组(Fe)、铁过载+椒目油组(Fe+ZBSO)。除SHAM组外,其余各组均实施卵巢切除术,Fe、Fe+ZBSO组以100 mg/kg·bw/w腹腔注射右旋糖酐铁,此外Fe+ZBSO组以3.46 ml/kg·bw/d干预12周。干预结束后,取股骨Micro-CT扫描骨结构和骨密度;ELISA法测定血清Ⅰ型胶原交联羧基端肽(CTXⅠ)、Ⅰ型前胶原氨基端原肽(PⅠNP)、8-羟基脱氧鸟苷(8-OHdG)、3-硝基酪氨酸(3-NT)的含量;比色法测定血清丙二醛(MDA)的浓度。结果Fe、Fe+ZBSO组相较于OVX组,血清铁蛋白含量上升(q=19.230,q=10.050,P<0.001);相较于SHAM组,OVX(Z=3.977,P<0.001)、Fe(Z=4.542,P<0.001)、Fe+ZBSO(Z=3.241,P=0.004)组子宫脏器指数降低。相较于Fe组,Fe+ZBSO组股骨结构更完整,骨密度上升(t=6.222,P<0.001);骨代谢结果显示,Fe+ZBSO组CTXⅠ降低(t=2.603,P=0.019)、PⅠNP升高(t=2.839,P=0.012);氧化损伤标志物结果显示,Fe+ZBSO组3-NT降低(t=2.894,P=0.015)、MDA降低(t=4.456,P<0.001),8-OHdG无差异(F=0.101,P=0.959)。结论椒目油能改善铁过载去势雌性SD大鼠的股骨骨骺端结构,提高骨密度,调节骨代谢,这可能是通过降低氧化损伤实现的。Objective To explore the protective effect of Zanthoxylum bungeanum Maxim seed oil(ZBSO)on iron overload-promoted osteoporosis in female ovariectomized SD rats and the possible mechanism.Methods 40 female SD rats were randomly divided into sham-operated group(SHAM),ovariectomized group(OVX),iron-overload group(Fe)and iron-overload+ZBSO group(Fe+ZBSO).Ovariectomy was performed in all groups except the SHAM group,and Fe and Fe+ZBSO groups were injected intraperitoneally with 100 mg/kg-bw/w of iron dextran,in addition to the Fe+ZBSO group with 3.46 mL/kg-bw/d of intervention for 12 weeks.After 12 weeks of intervention,the bone density and structure of femur were scanned by Micro-CT.The concentrations of type I collagen cross-linked carboxy-terminal peptide(CTXⅠ),type I precollagen amino-terminal protopeptide(PⅠNP),8-hydroxydeoxyguanosine(8-OHdG)and 3-nitrotyrosine(3-NT)were determined by ELISA.Colorimetric method was performed to determine serum malondialdehyde(MDA).Results Compared to the OVX group,the Fe and Fe+ZBSO groups showed an increase in serum ferritin content(q=19.230,q=10.050,P<0.001)and significant iron deposition in liver and bone.As opposed to the SHAM group,the OVX(Z=3.977,P<0.001),Fe(Z=4.542,P<0.001),and Fe+ZBSO(Z=3.241,P=0.004)groups showed a decrease in uterine organ index.Compared with the Fe group,the bone structure results showed that the Fe+ZBSO group had more intact femur structure and increased bone mineral density(t=6.222,P<0.001).Bone metabolism results showed that CTXⅠwas decreased(t=2.603,P=0.019),but PⅠNP was increased(t=2.839,P=0.012)in Fe+ZBSO group.Oxidative damage markers results demonstrated a decrease in 3-NT(t=2.894,P=0.015)and MDA(t=4.456,P<0.001),but no significant difference in 8-OHdG(F=0.101,P=0.959)in the Fe+ZBSO group compared to the Fe group.Conclusion ZBSO can improve femoral metaphyseal structure,increase bone density and regulate bone metabolism in ovariectomized female SD rats with iron overload,which may be achieved by reducing oxidative damage.

关 键 词：椒目油 铁过载 骨质疏松 骨代谢 氧化损伤 

分 类 号：R580[医药卫生—内分泌]