连翘酯苷A对脓毒症大鼠急性肺损伤的保护作用  

Protective effect of forsythiaside A on acute lung injury in septic rats

作　　者：蔡莉娟[1] 江荣林[2] Cai Lijuan;Jiang Ronglin(Department of Intensive Care Unit,the Third Affiliated Hospital of Zhejiang Chinese Medical University(Zhongshan Hospital of Zhejiang Province),Hangzhou 310005,Zhejiang,China;Department of Intensive Care Unit,the First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou 310006,Zhejiang,China)

机构地区：[1]浙江中医药大学附属第三医院(浙江省中山医院)ICU,杭州310005 [2]浙江中医药大学附属第一医院ICU,杭州310006

出　　处：《中华危重病急救医学》2022年第7期710-713,共4页Chinese Critical Care Medicine

基　　金：浙江省中医药科技计划项目(2015ZB073)。

摘　　要：目的观察连翘酯苷A对脓毒症大鼠急性肺损伤(ALI)的保护作用。方法将雄性SD大鼠随机分为正常对照组、假手术组、脓毒症模型组和连翘酯苷A干预组, 每组10只。正常对照组不予任何干预;假手术组仅行开关腹手术;模型组和连翘酯苷A干预组采用盲肠结扎穿孔术(CLP)制备脓毒症大鼠模型。连翘酯苷A干预组于术后0.5 h内灌胃连翘酯苷A 75 mL/kg, 6 h后重复灌胃1次;假手术组和模型组灌胃等量生理盐水。各组术后12 h取肺组织进行病理学观察;制备肺组织匀浆, 采用酶联免疫吸附试验(ELISA)检测肿瘤坏死因子-α(TNF-α)、白细胞介素(IL-1β、IL-6)水平;采用黄嘌呤氧化酶法检测超氧化物歧化酶(SOD)活性;采用比色法检测丙二醛(MDA)含量;采用蛋白质免疫印迹试验(Western blotting)检测核转录因子-κB p65(NF-κB p65)蛋白表达。结果正常对照组与假手术组肺组织均无明显病理学改变, 且各项指标差异均无统计学意义。模型组大鼠肺间质可见大量炎症细胞浸润, 肺泡结构破坏明显, 肺泡隔增宽, 肺泡腔内广泛出血, 毛细血管扩张;肺组织中TNF-α、IL-1β、IL-6、MDA含量及NF-κB p65蛋白表达均较正常对照组和假手术组显著升高〔TNF-α(ng/L):132.81±16.15比45.08±5.98、46.10±6.72, IL-1β(ng/L):137.32±15.22比51.03±7.89、50.92±8.13, IL-6(ng/L):138.39±14.28比51.68±7.03、52.48±7.36, MDA(kU/g):1.79±0.13比0.96±0.05、0.97±0.05, NF-κB p65蛋白(NF-κB p65/GAPDH):2.82±0.23比1.76±0.12、1.82±0.13, 均P<0.05〕, SOD活性则显著降低(kU/g:45.90±5.46比92.11±10.13、93.36±10.56, 均P<0.05)。连翘酯苷A干预组大鼠肺组织病理学改变较模型组明显改善, 表现为肺组织炎症细胞浸润明显减少, 肺泡隔增宽程度明显减小, 出血明显减少, 结构也较完整;肺组织TNF-α、IL-1β、IL-6、MDA含量及NF-κB p65蛋白表达均较模型组显著降低〔TNF-α(ng/L):72.48±9.78比132.81±16.15, IL-1β(ng/L):83.85�Objective To observe the protective effect of forsythiaside A on acute lung injury(ALI)in septic rats.Methods Male Sprague-Dawley(SD)rats were randomly divided into normal control group,sham operation group,sepsis model group,and forsythiaside A intervention group,with 10 rats in each group.The rats in the normal control group did not receive any intervention;the rats in the sham operation group only underwent abdominal surgery;and those in the model group and forsythiaside A intervention group received cecal ligation and puncture(CLP)to establish the sepsis rat model.The rats in the forsythiaside A intervention group were given 75 mL/kg of forsythiaside A within 0.5 hour after operation,and repeated after 6 hours.The rats in the sham operation group and model group were given the same amount of normal saline at the same time points.The lung tissues were collected for pathological examination 12 hours after operation.The lung homogenate was prepared,and enzyme-linked immunosorbent assay(ELISA)was used to detect tumor necrosis factor-α(TNF-α),interleukins(IL-1β,IL-6).The activity of superoxide dismutase(SOD)was detected by xanthine oxidase method,and the content of malonaldehyde(MDA)was detected by colorimetry.The expression of nuclear factor-κB p65(NF-κB p65)was detected by Western blotting.Results There was no significant pathological change of lung tissue in both normal control group and sham operation group,and there was no significant difference in each parameter between the two groups.The rats in the model group had interstitial infiltration of inflammatory cells,alveolar structure destruction,alveolar septum thicken,extensive alveolar hemorrhage,telangiectasia;the levels of TNF-α,IL-1β,IL-6,MDA and NF-κB p65 protein expression in lung tissue were significantly higher than those in the normal control group and sham operation group[TNF-α(ng/L):132.81±16.15 vs.45.08±5.98,46.10±6.72,IL-1β(ng/L):137.32±15.22 vs.51.03±7.89,50.92±8.13;IL-6(ng/L):138.39±14.28 vs.51.68±7.03,52.48±7.36;MDA(kU/g):1.

关 键 词：连翘酯苷A 脓毒症 肺损伤 

分 类 号：R285.5[医药卫生—中药学]