间接法建立反三碘甲状腺原氨酸生物参考区间  

Establishment of biological reference interval for reverse triiodothyronine by indirect method

作　　者：彰金[1] 李颖[1] 祁烁[2] 李伟强[1] 周通[1] Zhang Jin;Li Ying;Qi Shuo;Li Weiqiang;Zhou Tong(Department of Nuclear Medicine,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100700,China;Department of Thyroid,Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing 100700,China)

机构地区：[1]北京中医药大学东直门医院核医学科,北京100700 [2]北京中医药大学东直门医院甲状腺病科,北京100700

出　　处：《中华核医学与分子影像杂志》2022年第9期534-537,共4页Chinese Journal of Nuclear Medicine and Molecular Imaging

基　　金：北京中医药大学2018年度基本科研业务费项目(2018-JYBZZ-JS093)。

摘　　要：目的采用间接法建立适合本实验室的反三碘甲状腺原氨酸(rT3)生物参考区间。方法回顾性分析2019年4月至2019年9月间北京中医药大学东直门医院住院患者797例(男332例,女465例,年龄12~95岁),筛选甲状腺激素及相关抗体均正常且无甲状腺结节或明显甲状腺肿的人群,并进一步排除各种急慢性疾病、相关药物等影响rT3检测值的参考个体。rT3检测采用化学发光免疫分析法。筛选出的参考个体按年龄进行分组,采用两独立样本t检验、单因素方差分析和最小显著差异t检验分析数据。非参数排序法建立rT3生物参考区间,选取rT3数据分布的2.5%和97.5%百分位值作为上、下参考限的取值。采用简单随机抽样法抽取符合研究纳入及排除标准的健康体格检查者和住院患者各20例,验证参考区间。结果共有159例符合纳入及排除标准的参考个体入组,其中男66例,女93例,年龄23~87岁。23~29岁(4例)、30~39岁(18例)、40~49岁(29例)、50~59岁(43例)、60~69岁(40例)、70~79岁(19例)和80岁以上(6例)组的rT3值分别为(0.62±0.16)、(0.63±0.12)、(0.64±0.11)、(0.61±0.11)、(0.65±0.14)、(0.65±0.11)和(0.79±0.10)μg/L,不同年龄组rT3的差异有统计学意义(F=2.17,P=0.049),80岁以上组与其他各年龄组rT3差异均有统计学意义(均P<0.05),其余各组间差异均无统计学意义(均P>0.05)。80岁以下参考个体男性和女性的rT3值分别为(0.62±0.11)和(0.64±0.12)μg/L,差异无统计学意义(t=-0.81,P=0.420)。新建立适合20岁以上、80岁以下人群的rT3生物参考区间为0.47~0.92μg/L,与试剂说明书参考区间(0.20~0.95μg/L)比较,参考下限数值明显提高。20名健康体格检查者和20例住院患者rT3检测值分别为0.57~0.82μg/L、0.48~0.77μg/L,均在该参考范围内。结论本研究建立的rT3生物参考区间具有临床实际应用价值,但其年龄适用范围还需进一步完善。Objective To establish reverse triiodothyronine(rT3)biological reference interval suitable for laboratory by indirect method.Methods From April to September 2019,797 cases(332 males,465 females,age:12-95 years)underwent thyroid function,thyroid related antibody and rT3 tests from hospitalized population in Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine were retrospectively analyzed.The reference individuals with normal thyroid hormone,antibody and without thyroid nodule or goiter were screened as inclusion criteria,and the factors such as acute and chronic diseases or drugs that might affect the values of rT3 were excluded.Independent sample t test,one-way analysis of variance and least significant difference t test were used to analyze data.The rT3 reference interval was established by non-parametric sequencing method,and 2.5%and 97.5%percentile values of data distribution were selected as the upper and the lower reference limits.In order to verify the rT3 reference interval,20 healthy individuals and 20 inpatients who met the inclusion and exclusion criteria were selected to test rT3 with a simple random sampling method.Results A total of 159 reference individuals(66 males,93 females,age:23-87 years)were enrolled.The rT3 values of 23-29(n=4),30-39(n=18),40-49(n=29),50-59(n=43),60-69(n=40),70-79(n=19)and over 80(n=6)years old groups were(0.62±0.16),(0.63±0.12),(0.64±0.11),(0.61±0.11),(0.65±0.14),(0.65±0.11)and(0.79±0.10)μg/L,respectively.There was a statistically significant difference in the rT3 test results among different age groups(F=2.17,P=0.049).There were statistically significant differences of rT3 between the individuals over 80 years old and other age groups(all P<0.05),while there were no statistically significant differences among the other groups(all P>0.05).The rT3 of males and females under 80 years old were(0.62±0.11)and(0.64±0.12)μg/L,respectively,with no significant difference between them(t=-0.81,P=0.420).The newly established rT3 reference interval suitabl

关 键 词：三碘甲状腺原氨酸 逆转 化学发光测定法 参考值 

分 类 号：R446.1[医药卫生—诊断学]