高氨氮沼液浸种强化小球藻对沼液废水的处理效果  

Treatment effect of enhanced Chlorella by high ammonia nitrogen biogas slurry seed soaking on biogas slurry wastewater

作　　者：谭顺 莫创荣 许雪棠[2] 庞瑞林 王露洁 陆祖逸 TAN Shun;MO Chuang-rong;XU Xue-tang;PANG Rui-lin;WANG Lu-jie;LU Zu-yi(School of Resources,Environment and Materials,Guangxi University,Nanning 530004,China;School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China)

机构地区：[1]广西大学资源环境与材料学院,南宁530004 [2]广西大学化学化工学院,南宁530004

出　　处：《西南农业学报》2022年第8期1786-1793,共8页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金(22065003)。

摘　　要：【目的】探究高氨氮沼液浸种对小球藻生长状况及浸种小球藻对沼液处理效果的影响,为利用强化小球藻解决沼液污染物提供参考依据。【方法】使用高氨氮(1640.0 mg/L)沼液直接浸种小球藻0、4、8和12 h,比较不同浸种时间小球藻在BG11培养基和含氨氮培养基中的生长状况、藻细胞中的总光合色素浓度及叶绿素a、叶绿素b和类胡萝卜素含量差异,测定分析最佳浸种时间小球藻在实际废水中的生长状况和脱氮除磷效果。【结果】在BG11培养基中,浸种时间越长的小球藻生长受到抑制作用越明显,恢复正常生长所需时间越长;其光合色素损伤越重,叶绿素a/b下降越明显,叶绿素a/b达到峰值所需时间越长。在含氨氮培养基中,浸种8 h小球藻具有更高的相对生长量和更强的氨氮吸收同化能力;培养7 d后,浸种8 h小球藻的OD值较未浸种小球藻提高17.3%,叶绿素a含量也最高,是未浸种小球藻的1.17倍。在实际废水培养基中培养7 d后,浸种8 h小球藻的OD值比未浸种小球藻提高26.0%,氨氮去除率较未浸种小球藻提高13.1%;浸种8 h小球藻继承了未浸种小球藻的高效除磷性,培养3 d后,2种小球藻对实际废水中总磷的去除率均在95.0%以上。【结论】利用高氨氮沼液浸种小球藻8 h对小球藻的强化效果最佳,经强化的小球藻叶绿素a和生物量积累更多,脱氮除磷效果较佳,可在实际废水处理中推广应用。【Objective】The present paper aimed to explore the effect of biogas slurry with high ammonia and nitrogen soaking on the growth of Chlorella and the effect of soaking Chlorella on the treatment of biogas slurry, so as to provide a reference for the use of enhanced Chlorella to solve biogas slurry pollutants.【Method】Chlorella was directly soaked with(1640.0 mg/L) biogas slurry with high ammonia and nitrogen for 0,4,8 and 12 hours, and then the growth status of Chlorella in BG11 medium and ammonia nitrogen medium and the difference in total photosynthetic pigment concentration and chlorophyll a, chlorophyll b and carotenoid contents in algal cells were compared at different soaking times, and determine and analyze the growth status and nitrogen and phosphorus removal effects of Chlorella in actual wastewater at the best soaking time.【Result】In BG11 medium, the longer the soaking time of Chlorella,the more obvious the inhibition of its growth and the longer it took to restore normal growth;The heavier its photosynthetic pigment damage, the more pronounced the decrease in chlorophyll a/b and the longer it took for chlorophyll a/b to reach its peak. In the medium containing ammonia nitrogen, 8 hours seed soaking Chlorella had higher relative growth and stronger ammonia nitrogen absorption and assimilation ability;After 7 days of incubation, the OD value of 8 hours seed soaking Chlorella increased by 17.3% compared to that of unsoaked Chlorella,and the chlorophyll a content of 8 hours seed soaking Chlorella was also the highest, 1.17 times higher than that of unsoaked Chlorella. After 7 days of incubation in actual wastewater medium, the OD value of 8 hours seed soaking Chlorella increased by 26% compared with that of unsoaked Chlorella,and the ammonia nitrogen removal rate was increased by 13.1% than unsoaked Chlorella;8 hours seed soaking Chlorella inherited the efficient phosphorus removal of unsoaked Chlorella,and the removal rate of total phosphorus from the actual wastewater by both types of Chlorella

关 键 词：高氨氮沼液 小球藻 浸种 生长状况 叶绿素A 脱氮除磷效果 

分 类 号：S154.38[农业科学—土壤学]