5-羟色胺和氨海水对钝缀锦蛤解剖卵母细胞的体外促熟作用  

Effect of 5-HT and ammonia seawater on maturation of oocyte stripped from Tapes conspersus

作　　者：巫旗生[1] 祁剑飞[1] 宁岳[1] 罗娟[1] 庞小鹏 郭香[1] 罗辉玉 曾志南[1] WU Qisheng;QI Jianfei;NING Yue;LUO Juan;PANG Xiaopeng;GUO Xiang;LUO Huiyu;ZENG Zhinan(Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province,Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources,National and Local Joint Engineering Research Center of Marine Biological Seed Industry Technology,Fisheries Research Institute of Fujian,Xiamen 361013,China;Fisheries College,Jimei University,Xiamen 361021,China)

机构地区：[1]福建省水产研究所,福建省海洋生物增养殖与高值化利用重点实验室,福建省海洋生物资源开发利用协同创新中心,海洋生物种业技术国家地方联合工程研究中心,福建厦门361013 [2]集美大学水产学院,福建厦门361021

出　　处：《渔业研究》2022年第5期477-483,共7页Journal of Fisheries Research

基　　金：现代农业产业技术体系建设专项(CARS-49);国家海洋水产种质资源库项目;福建省省属公益类科研院所基本科研专项(2019R1013-12)。

摘　　要：采用解剖卵母细胞体外浸泡促熟的方法,研究了5-羟色胺(5-HT)和氨海水对钝缀锦蛤(Tapes conspersus)卵母细胞的促熟作用。结果表明,采用一定浓度的5-HT和氨海水浸泡均能够显著诱导钝缀锦蛤卵母细胞的生发泡破裂(P<0.05),5-HT和氨海水浸泡的卵母细胞受精率相差不大(P>0.05)。其中,10μmol/L 5-HT浸泡卵母细胞60 min获得的受精率最高,达到48.57%;0.020%氨海水浸泡卵母细胞45 min获得的受精率最高,达到50.75%,但畸形较多。To construct the artificial breeding method of Tapes conspersus,effects of 5-hydroxytryptamine(5-HT)and ammonia seawater on the maturation of oocytes were studied by soaking the dissected oocytes in vitro.Results showed that both immersion with ammonia seawater and 5-hydroxytryptamine significantly increase the germinal vesicles breakdown in Tapes conspersus(P<0.05).However, the fertilization rate showed no significantly different between oocytes soaked in 5-hydroxytryptamine and ammonia seawater.The fertilization rate of oocytes treated with 10 μmol/L 5-HT for 60 min was 48.57%.The fertilization rate of oocytes treated with 0.020% ammonia seawater for 45 min was slightly higher(about 50.75%),whereas there were many malformations.

关 键 词：钝缀锦蛤 卵母细胞 体外促熟 5-羟色胺 氨海水 

分 类 号：S968.31[农业科学—水产养殖]