南雄辣椒炭疽病菌的分离鉴定与药剂筛选  

Isolation,Identification and Fungicide Screening of Capsicum Anthracnose Pathogen in Nanxiong County

作　　者：郑婕 庄远航 郭梓琪 许艺 钟俊杰 黄维峰 胡芳 李海渤[1] 雷建军 吴昊[1] ZHENG Jie;ZHUANG Yuanhang;GUO Ziqi;XU Yi;ZHONG Junjie;HUANG Weifeng;HU Fang;LI Haibo;LEI Jianjun;WU Hao(Henry Fok College of Biology and Agriculture,Shaoguan University,Shaoguan 512005,Guangdong,China;College of Horticulture,South China Agricultural University,Guangzhou 510642,Guangdong,China;Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region,ShaoGuan University,Shaoguan 512005)

机构地区：[1]韶关学院英东生物与农业学院,广东韶关512005 [2]华南农业大学园艺学院,广东广州510642 [3]广东省粤北食药资源利用与保护重点实验室,广东韶关512005

出　　处：《中国蔬菜》2022年第10期58-65,共8页China Vegetables

基　　金：国家自然科学基金项目(32000233);韶关市科技计划项目(210726124532350);南雄市科技特派员项目;2021年韶关学院大学生创新创业训练计划项目(Sycxcy2021148)。

摘　　要：为明确广东省韶关市南雄市辣椒炭疽病病原菌种类,并筛选能有效抑制该病原菌的杀菌剂,对采集自南雄市湖口镇露地朝天椒的疑似炭疽病自然发病辣椒果实,利用组织分离法分离纯化炭疽病病原菌,微生物形态学结合多基因系统进化树分析鉴定病菌种类,并通过室内毒力测定试验筛选能够高效防治该病菌的杀菌剂。结果表明,所分离菌株NX-1的病菌种类为Colletotrichum scovillei;12种杀菌剂中,咪鲜胺和苯甲·丙环唑对该病菌的抑制作用最强,EC_(50)分别为0.0196μg·mL^(-1)和0.2828μg·mL^(-1)。咪唑类杀菌剂咪鲜胺可作为南雄辣椒炭疽病防治药剂的首选,或与苯甲·丙环唑交替使用。In order to clarify the pathogen species of pepper anthracnose in Nanxiong County,Shaoguan City,Guangdong Province and screen fungicides that can effectively inhibit the pathogen,this study isolated and purified the anthrax pathogen from the fruits of Chaotian pepper with suspected natural anthracnose sympton,collected from open fields of Hukou Town,Nanxiong County by tissue separation method,and the pathogen species were identified by microbial morphology combined with multi-gene phylogenetic tree analysis.Then,fungicides that could efficiently control this pathogen were screened by indoor toxicity test.The results showed that the pathogen of the isolated strain NX-1 was Colletotrichum scovillei.Among 12 fungicides,prochloraz and difenoconazole·propiconazole had the strongest inhibitory effect on the pathogen,with EC_(50) values of 0.0196μg·mL^(-1) and 0.2828μg·mL^(-1),respectively.Imidazole fungicide prochloraz can be used as the first choice chemist for controlling pepper anthracnose in Nanxiong,or be used alternatively with benzyl propiconazole.

关 键 词：辣椒炭疽病 病原菌鉴定 室内毒力测定 

分 类 号：S436.418.11[农业科学—农业昆虫与害虫防治]