低温胁迫下不同耐低温性中国南瓜苗期生理差异分析  

Analysis of Physiological Differences in Seedling Stage of Cucurbita moschata Duch.with Different Cold Tolerance under Low Temperature Stress

作　　者：邱喜岩 段颖[1] 张硕[1] 张蒙 疏琴 孙雅佩 何亚迪 廖青桂 王长林[1,2] 薄凯亮 QIU Xiyan;DUAN Ying;ZHANG Shuo;ZHANG Meng;SHU Qin;SUN Yapei;HE Yadi;LIAO Qinggui;WANG Changlin;BO Kailiang(Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural Affairs,Beijing 100081,China;China Vegetable Biotechnology(Shouguang)Co.,Ltd,Shouguang 262700,Shandong,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,农业农村部园艺生物学与种质创制重点实验室,北京100081 [2]中蔬生物科技(寿光)有限公司,山东寿光262700

出　　处：《中国蔬菜》2022年第10期74-80,共7页China Vegetables

基　　金：北京市设施蔬菜创新团队项目(BAIC01);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS);中央级公益性科研院所基本科研业务费资助项目(IVF-BRF2021007)。

摘　　要：以2份耐低温和2份低温敏感的中国南瓜幼苗为试材,在人工气候室中进行昼/夜为10℃/2℃(12 h/12 h)的低温胁迫处理,以昼/夜为25℃/15℃(12 h/12 h)作为常温对照,研究低温胁迫对不同耐低温性中国南瓜幼苗生理指标和光合作用相关指标的影响。结果表明:低温胁迫10 d时,耐低温种质的可溶性糖含量仍明显高于低温敏感种质;而低温胁迫后的可溶性蛋白含量在耐低温种质与低温敏感种质间无明显差异;耐低温种质能够在低温胁迫下更好的维持叶片叶绿素含量,且净光合速率明显高于低温敏感种质。相关性分析表明,Fv/Fm与Fv/F_(0)呈显著正相关,与气孔导度呈显著负相关;净光合速率、叶绿素含量和可溶性糖含量之间均表现为显著或极显著的正相关关系。以上结果表明,低温胁迫7 d时,可溶性糖含量、叶绿素含量和净光合速率可以作为中国南瓜种质耐低温性鉴定的可靠指标。Taking 2 low-temperature tolerant and 2 low-temperature sensitive Cucurbita moschata Duch.seedlings as test materials,this experiment conducted low-temperature stress treatment:with day and night 10℃/2℃(12 h/12 h)in an artificial climate chamber;took day and night 25℃/15℃(12 h/12 h)as a normal temperature contrast;and studied the effects of low-temperature stress on physiological indexes and photosynthetic related indexes of Cucurbita moschata seedlings with different low temperature tolerance.The results indicated that when low-temperature stress sustained 10 days,the soluble sugar content in low-temperature tolerant germplasm resources was still obviousely higher than that in low-temperature sensitive germplasm resources.While,after low-temperature stress,there were no obviouse differences among soluble protein content in low-temperature tolerant germplasm resources and low-temperature sensitive germplasm resources.The low-temperature tolerant germplasm resources could better maintain the chlorophyll content in leaves,and its net photosynthetic rate was signifigantly higher than that in low-temperature sensitive germplasm resources.Correlation analysis showed that Fv/Fm was significantly positively correlated with Fv/F_(0) and negatively correlated with stomatal conductance.The net photosynthetic rate,chlorophyll content and soluble sugar content showed significant or extremely significant positive correlation.The above results indicated that the soluble sugar content,chlorophyll content and net photosynthetic rate could be used as reliable indexes to identify the lowtemperature tolerance of Cucurbita moschata germplasm resources under low temperature stress for 7 days.

关 键 词：中国南瓜 低温胁迫 生理指标 光合作用 

分 类 号：S642.1[农业科学—蔬菜学]