三株耐盐菌的筛选及蜡样芽孢杆菌对Cr(Ⅵ)的去除效应研究  

Screening of three salt-tolerant bacteria and removal effect of Bacillus cereus on Cr(Ⅵ)

作　　者：王超 徐静 张前熙 WANG Chao;XU Jing;ZHANG Qian-xi(Sichuan Vocational College of Health and Rehabilitation,Zigong 643000,Sichuan,China)

机构地区：[1]四川卫生康复职业学院,四川自贡643000

出　　处：《湖北农业科学》2022年第18期29-34,共6页Hubei Agricultural Sciences

基　　金：四川省自然科学基金资助项目(2022NSFSC1785);四川省自贡市重点科技计划基金资助项目(2020YGJC26);四川卫生康复职业学院院级重点科研基金资助项目(CWKY-2019Z-06);四川卫生康复职业学院生物资源开发与利用创新团队资金资助项目(CWKY-TD20-06)。

摘　　要：为挖掘高盐环境下Cr(Ⅵ)废水处理的微生物资源,以四川自贡长山盐矿地下卤水及周边环境土壤为材料,分离纯化出3株耐盐菌N1、N2、N3,经16S测序鉴定为蜡样芽孢杆菌(Bacillus cereus)、高地芽孢杆菌(Bacillus altitudinis)、希瓦氏菌(Shewanella sp.)。3株菌株在不同NaCl浓度下生长时均表现出低浓度促进、高浓度抑制的趋势,NaCl浓度为10%时,N1、N2、N3菌株均正常生长;当NaCl浓度达15%时,N1菌株可正常生长,N2、N3菌株生长受到不同程度的抑制,耐盐能力为N1>N2>N3。选取耐盐能力最佳的N1菌株为供试菌株用于研究Cr(Ⅵ)的去除效应,在NaCl浓度为10%、Cr(Ⅵ)浓度为40 mg/L条件下,72 h后Cr(Ⅵ)的去除率达到81.2%,去除速率与菌体浓度呈正相关。In order to explore the microbial resources for Cr(Ⅵ)wastewater treatment under high salt conditions,the underground brine and surrounding soil of Changshan salt mine in Zigong,Sichuan was taken as the research materials in this experiment.Three strains of salt tolerant bacteria N1,N2 and N3 were isolated and purified,identified as Bacillus cereus,Bacillus altitudinis and She⁃wanella sp.after 16S sequencing.The trend of low concentration promotion and high concentration inhibition was showed when the three strains grew at different NaCl concentrations N1,N2 and N3 strains grew normally under 10%NaCl concentration;when the Na⁃Cl concentration reached 15%,N1 strain could grow normally.At the same time,the growth of N2 and N3 strains was inhibited to vary⁃ing degrees.The results reflected that the salt tolerance of strains was N1>N2>N3.N1 strain with the best salt tolerance was selected as the test strain to study the removal effect of Cr(Ⅵ).When concentration of NaCl was 10%and Cr(Ⅵ)was 40 mg/L,the removal rate of Cr(Ⅵ)reached 81.2%after 72 h.The removal speed of Cr(Ⅵ)was positively correlated with cell concentration.

关 键 词：微生物修复 Cr(Ⅵ) 嗜盐微生物 蜡样芽孢杆菌 耐盐 

分 类 号：Q939.9[生物学—微生物学]