低温保存时间对吉林马精液活力及母马情期受胎率的影响  

Effects of cryopreservation time on semen motility and estrus conception rate in Jilin horses

作　　者：刘晓辉[1] 郎洪彦[1] 谭成成 张鹏举[1] 王伟霞 朴庆林[1] 李信涛[1] LIU Xiao-hui;LANG Hong-yan;TAN Cheng-cheng;ZHANG Peng-ju;WANG Wei-xia;PU Qing-lin;LI Xin-tao(Institute of Animal Biotechnology,Jilin Academy of Agricultural Sciences,Gongzhuling 136100,Jilin,China)

机构地区：[1]吉林省农业科学院动物生物技术研究所,吉林公主岭136100

出　　处：《湖北农业科学》2022年第18期155-158,共4页Hubei Agricultural Sciences

基　　金：吉林省农业科技创新工程项目(CXGC2018ZY008);吉林省科技发展地方科技创新引导与扶贫项目(20200702044NC)。

摘　　要：为研究低温保存时间对吉林马精液运动特性及母马情期受胎率的影响,试验采用柠檬酸-果糖-Tris-甘油-卵黄为稀释液,试验组取新鲜精液及在低温(4℃)条件下保存24、48、72、96、120 h的精液,检测精子的运动特性,并统计150匹吉林马母马人工授精后的情期受胎率。结果表明,与新鲜精液相比,运动精子活力、前向运动精子活力、平均运动速率及快速运动精子活力随着保存时间的延长而降低。精液在低温(4℃)条件下保存0、24、48、72 h,人工授精后吉林马母马情期受胎率分别为43.33%、44.00%、44.45%、43.48%,差异不显著(P>0.05),而保存96 h和120 h,人工授精后吉林马母马情期受胎率分别为36.00%、10.00%,差异显著(P<0.05),表明吉林马精液在低温(4℃)条件下有效保存时间不能超过72 h。In order to study the effect of cryopreservation time on the semen motility characteristics of Lin horses and the conception rate of mares during estrus,the experiment used citric acid-fructose-Tris-glycerol-yolk as the diluent.In the experimental group,fresh semen and semen stored at low temperature(4℃)for 24,48,72,96,and 120 h were collected,and the sperm motility charac⁃teristics were detected by conventional sperm quality testing methods.The results showed that compared with fresh sperm,motile sperm motility,forward motility sperm motility,average motility rate and fast motility sperm motility decreased with the prolongation of storage time.When the semen was stored at low temperature(4℃)for 0,24,48,and 72 h,the estrus conception rates of Jilin horse mares after artificial insemination were 43.33%,44.00%,44.45%,and 43.48%,respectively,with no significant difference(P>0.05).However,after 96 h and 120 h storage,the estrus conception rates of Jilin horse mares after artificial insemination were 36.00%and 10.00%,respectively(P<0.05),indicating that the effective storage time of Jilin horse semen at low temperature(4℃)cannot exceed 72 h.

关 键 词：吉林马 低温 精子活力 人工授精 情期受胎率 

分 类 号：S821.3[农业科学—畜牧学]