酶法辅助乙醇提取废次烟叶绿原酸的工艺研究  

Study on the extraction process of chlorogenic acid from discarded tobacco leaves by ethanol assisted with enzyme

作　　者：陈月星 黄金辉 阎佩云 张晓虎 CHEN Yue-xing;HUANG Jin-hui;YAN Pei-yun;ZHAGN Xiao-hu(School of Biomedicine and Food Engineering,Shangluo University,Shangluo 726000,Shaanxi,China;Shaanxi Qinling Characteristic Biological Resources Industry Technology Research Institute,Shangluo 726000,Shaanxi,China;Luonan County Branch of Shangluo City Tobacco Company,Shaanxi Tobacco Company,Shangluo 726100,Shaanxi,China)

机构地区：[1]商洛学院生物医药与食品工程学院,陕西商洛726000 [2]陕西秦岭特色生物资源产业技术研究院,陕西商洛726000 [3]陕西省烟草公司商洛市公司洛南县分公司,陕西商洛726100

出　　处：《湖北农业科学》2022年第18期180-184,共5页Hubei Agricultural Sciences

基　　金：商洛市科技计划项目(SK2019-70);中央引导地方科技发展专项(2019ZY-FP-02);商洛学院科研创新团队项目(19SXC03);商洛学院人才引进项目(17SKY015)。

摘　　要：以废次烟叶为原料,应用酶法辅助乙醇法对烟叶中的绿原酸进行提取,通过单因素试验分析浸泡温度、浸泡时间、浸提温度、料液比4个因素对绿原酸提取率的影响,利用响应面设计对提取工艺进行优化。结果表明,最终优化工艺参数为浸泡温度60℃,浸泡时间1 h,浸提温度90℃,料液比1∶25(m∶V),废次烟叶中绿原酸提取率为25.709 mg/g,与模型模拟得到的25.863 mg/g基本一致。Taking discarded tobacco leaves as raw material,the chlorogenic acid was extracted from tobacco leaves by ethanol assisted with enzyme.Single factor experiment was conducted to investigate the effects of soaking temperature,soaking time,extraction temper⁃ature,and solid-liquid ratio on chlorogenic acid extraction rate.On this basis,the extraction process was optimized by the response surface experiments.The results showed that the optimized parameters were as follows:soaking temperature 60℃,soaking time 1 h,leaching temperature 90℃,and solid-liquid ratio 1∶25(m∶V).Under the above process,the chlorogenic acid extraction rate was 25.709 mg/g,which was consistent with the model predicted value of 25.863 mg/g.

关 键 词：废次烟叶 绿原酸 酶法辅助乙醇 响应面 

分 类 号：S572[农业科学—烟草工业]