海南黎族人群5个血型系统基因筛检及稀有血型库的建立  

Genetic screening of 5 blood group systems of Li ethnic group in Hainan province and establishment of rare blood group bank

作　　者：符小玲 蔡兴权[2] 夏兰[2] FU Xiao-ling;CAI Xing-quan;XIA Lan(Department of Blood Transfusion,Hainan Women and Children’s Medical Center,Haikou 570311,China;Department of Blood Transfusion,Hainan General Hospital,Haikou 570311,China)

机构地区：[1]海南省妇女儿童医学中心输血科,海南海口570311 [2]海南省人民医院输血科,海南海口570311

出　　处：《海南医学院学报》2022年第20期1526-1532,1539,共8页Journal of Hainan Medical University

基　　金：海南省自然科学基金青年基金项目(820QN410);海南省临床医学中心建设项目资助[琼卫医函(2021)75号]。

摘　　要：目的:对海南黎族人群Duffy、Lutheran、Kidd、Diego、Dombrock血型系统基因进行筛检,为本地区稀有血型资料库提供实验室依据。方法:采用PCR-SSP的方法对300份海南黎族人群志愿者Duffy、Lutheran、Kidd、Diego、Dombrock血型系统等位基因进行检测和多态性分析。结果:海南黎族人群Duffy、Lutheran、Kidd、Diego、Dombrock血型系统等位基因频率分别是:Fy^(a)=0.9583、Fy^(b)=0.0417;Au^(a)=0.8350、Au^(b)=0.1650;Jk^(a)=0.4500、Jk^(b)=0.5500;Di^(a)=0.0667、Di^(b)=0.9333;Do^(a)=0.1017、Do^(b)=0.8983。Duffy、Lutheran、Kidd、Diego、Dombrock血型系统等位基因对偶抗原Fy^(a)/Fy^(b)、Au^(a)/Au^(b)、Jk^(a)/Jk^(b)、Di^(a)/Di^(b)、Do^(a)/Do^(b)的不配合率分别为:7.67%、23.76%、37.25%、11.67%、16.60%。结论:海南黎族人群的Duffy、Lutheran、Kidd、Diego、Dombrock血型系统基因频率分布呈现多态性,等位基因对偶抗原不配合率较高,与我国其他不同民族比较,存在较大的差异,具有独特的民族分布特征,对本地区稀有血型库的建立具有重要意义。Objective:To screen the blood group system genes of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic group in Hainan Province and provide laboratory data for the rare blood group database in this area.Methods:The alleles of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of 300 voluntary participants of Li ethnic group in Hainan were detected by sequence-specific primer polymerase chain reaction,and the polymorphism was analyzed.Results:The allele frequencies of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic groups in Hainan Province are 0.9583 for Fy^(a),0.0417 for Fy^(b),0.8350 for Au^(a),0.1650 for Au^(b),0.4500 for Jk^(a),0.5500 for Jk^(b),0.0667 for Di^(a),0.9333 for Di^(b),0.1017 for Do^(a) and 0.8983 for Do^(b),respectively.The antigen incompatibility rates of Fy^(a)/Fy^(b),Au^(a)/Au^(b),Jk^(a)/Jk^(b),Di^(a)/Di^(b),Do^(a)/Do^(b) of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems were 7.67%,23.76%,37.25%,11.67%and 16.60%,respectively.Conclusion:The gene frequencies of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic group in Hainan Province are polymorphic,and the antigen incompatibility rates of alleles are higher,which is quite different from that of other nationalities in China and with unique ethnic distribution characteristics.It is of great significance to establish the rare blood group database in this region.

关 键 词：血型基因 基因频率 稀有血型库 黎族 

分 类 号：R457.11[医药卫生—治疗学]