碳源对白桦茸菌丝体生长及多糖活性的影响  

Effects of Carbon Sources on Mycelial Growth and Polysaccharide Activity of Inonotus obliquus

作　　者：秦锦涛 王亮 曹羽杰 赖玉婷 郑钦华 林翰 黄卓欣 杨东生 王兰英 QIN Jin-tao;WANG Liang;CAO Yu-jie;LAI Yu-ting;ZHENG Qin-hua;LIN Han;HUANG Zhuo-xin;YANG Dong-sheng;WANG Lan-ying(School of Pharmacy and Food Science,Zhuhai College of Science and Technology,Zhuhai 519041,Guangdong,China)

机构地区：[1]珠海科技学院药学与食品科学学院,广东珠海519041

出　　处：《食品研究与开发》2022年第21期51-59,共9页Food Research and Development

基　　金：广东大学生科技创新培育专项资金“攀登计划”项目(Pdjh2020b0764);2019年全国大学生创新创业项目(S201913684035);2019年广东省创新强校工程项目(2019KTSCX222、2019KQNCX200)。

摘　　要：以白桦茸为研究对象,采用平板培养及液体摇瓶培养的方式,观察、比较不同碳源(麦芽糖、葡萄糖、蔗糖、玉米淀粉、乳糖、玉米面粉、果糖、甘露醇、红薯淀粉、可溶性淀粉)对菌丝部分生物学特性及液体发酵产物活性的影响。固体培养结果表明:果糖、麦芽糖、蔗糖对菌丝生长速度影响较大,麦芽糖为碳源时,菌丝生长速度最快,达到0.302 cm/d,果糖和蔗糖可以延缓菌丝老化,而玉米面粉和葡萄糖的菌丝最为粗壮,麦芽糖和红薯淀粉的菌丝生长密度最高(密集处呈羊毛毡状);同时,液体发酵结果表明:玉米面粉总生物量最大,葡萄糖次之。对于活性物质来说,随着培养时间的延长,大部分碳源均可促进活性物质的积累,以葡萄糖为碳源,11 d菌丝体多糖含量最高,其次为乳糖。红薯淀粉、玉米淀粉、麦芽糖、乳糖为碳源时,其菌丝水提液1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl radical,DPPH)自由基清除率可达到70%以上。Taking Inonotus obliquus as the research object,this research adopted the way of plate culture and liquid shake flask culture to observe and compare the effects of different carbon sources(maltose,glucose,sucrose,corn starch,lactose,corn flour,fructose,mannitol,sweet potato starch and soluble starch)on the biological characteristics of mycelium and the activity of liquid fermentation products. The results of solid culture showed that fructose,maltose and sucrose had a great influence on the growth rate of mycelium. When maltose was the carbon source,the mycelium grew fastest,reaching 0.302 cm/d. Fructose and sucrose delayed the aging of mycelium,the mycelia of corn flour and glucose were the strongest,and the mycelia of maltose and sweet potato starch had the highest growth density(the dense part was felt like wool shaped). At the same time,the results of liquid fermentation showed that the total biomass of corn flour was the largest,followed by glucose.As for the active substances,most carbon sources promoted the accumulation of active substances with the extension of culture time. With glucose as the carbon source,the polysaccharide content of mycelium was the highest at 11 d,followed by lactose. When sweet potato starch,corn starch,maltose,and lactose were used as carbon sources,the 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging rate of water extract of mycelium reached more than 70%.

关 键 词：抗氧化 白桦茸 碳源 液体发酵 生物学特性 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]