限制性因子黏液病毒抗性蛋白Ⅱ对乙型肝炎病毒复制的影响  

Inhibition of Restriction Factor Myxovirus Resistance ProteinⅡon Hepatitis B Virus Replication

作　　者：代娟 胡杰[2] 彭胡 Dai Juan;Hu Jie;Peng Hu(Department of Laboratory Medicine,Chongqing Jiulongpo District Hospital of Traditional Chinese Medicine,Chongqing 400080,China;Chongqing Medical University,Chongqing 400016,China;Department of Laboratory Medicine,Luzhou Hospital of Traditional Chinese Medicine,Luzhou 646000,China)

机构地区：[1]重庆市九龙坡区中医院检验科,重庆400080 [2]重庆医科大学,重庆400016 [3]泸州市中医医院检验科,泸州646000

出　　处：《成都医学院学报》2022年第5期597-601,共5页Journal of Chengdu Medical College

基　　金：重庆医科大学感染性疾病分子生物学教育部重点实验室开放基金项目(No:202102);西南医科大学-泸州市中医医院基地基金项目(No:2019-LH001)。

摘　　要：目的研究干扰素α(IFNα)对限制性因子黏液病毒抗性蛋白Ⅱ(MX2)的诱导作用,以及MX2对乙型肝炎病毒(HBV)复制的影响。方法使用不同剂量的IFNα处理肝癌细胞HepG2、含有牛磺胆酸钠协同转运肽(NTCP)的HepG2细胞(简称HepG2-NTCP细胞);采用荧光定量聚合酶链式反应(PCR)、蛋白质印迹技术检测MX2在转录或翻译后的表达水平;采用DNA印迹技术及qPCR检测过表达mx2对HBV DNA和HBV前基因组RNA(pgRNA)的抑制作用;通过siRNA敲低内源性的mx2,DNA印迹技术检测其对HBV病毒复制的影响。结果在HepG2和HepG2-NTCP细胞中,经IFNα处理后,MX2的蛋白及mRNA水平升高(P<0.05);过表达mx2后,其HBV DNA和HBV pgRNA水平均降低(P<0.05)。结论在HepG2、HepG2-NTCP细胞中,IFNα可诱导MX2表达上调,并且过表达MX2可抑制HBV的复制。Objective To study restriction factor myxovirus resistance proteinⅡ(mx2)expression induced by interferonα(IFNα),and the inhibition of hepatitis B virus(HBV)replication by mx2.Methods HepG2 or HepG2 cells containing sodium taurocholate cotransporting peptide(NTCP)(referred to as HepG2-NTCP cells)were treated with different doses of IFNα.The transcription and translation levels of mx2 were detected by real-time quantitative polymerase chain reaction(PCR)or Western blot.The inhibition of mx2 overexpression on HBV DNA replication and HBV pregenomic RNA(pgRNA)level was detected by Southern blot or qPCR.The endogenous mx2 was knocked down by small interfering Ribonucleic Acid(siRNA),and Southern blot was used to detect its effect on HBV replication.Results In HepG2 and HepG2-NTCP cells,the mRNA levels and protein expression of mx2 were significantly increased after the treatment of IFNα(P<0.05).Over expression of mx2 significantly inhibited HBV DNA replication and HBV pgRNA levels.Conclusion In HepG2 and HepG2-NTCP cells,IFNαcan induce upregulation of MX2 expression,and overexpression of mx2 significantly inhibits HBV replication.

关 键 词：乙型肝炎病毒 干扰素 黏液病毒抗性蛋白Ⅱ 抑制 

分 类 号：R373.21[医药卫生—病原生物学]